We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
EnGen® Sau Cas9 is a programmable DNA endonuclease guided by a single guide RNA (gRNA). Targeting requires gRNA complementarity to the target site as well as a 5′NNGRRT protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen® Sau Cas9 occurs ~3 bases upstream of the PAM and leaves blunt ends.
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