HomeFAQsHow can the genome editing efficiency be determined following EnGen® Sau Cas9 (NEB #M0654) transfections or microinjections?
FAQ: How can the genome editing efficiency be determined following EnGen® Sau Cas9 (NEB #M0654) transfections or microinjections?
T7 Endonuclease I (NEB #M0302) or the EnGen Mutation Detection Kit (NEB #E3321S) can be used to determine editing events generated by Sau Cas9 RNP transfections or microinjections. Other methods, including amplicon sequencing, can also be used for analysis of editing.
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