FAQ: How can the genome editing efficiency be determined following EnGen® Sau Cas9 (NEB #M0654) transfections or microinjections?

T7 Endonuclease I (NEB #M0302) or the EnGen Mutation Detection Kit (NEB #E3321S) can be used to determine editing events generated by Sau Cas9 RNP transfections or microinjections.  Other methods, including amplicon sequencing, can also be used for analysis of editing.