HomeFAQsWhy do I observe incomplete digestion with EnGen® Sau Cas9 and my target-specific Sau sgRNA in vitro?
FAQ: Why do I observe incomplete digestion with EnGen® Sau Cas9 and my target-specific Sau sgRNA in vitro?
The efficiencies of sgRNAs vary depending on the specific target sequences. We recommend using an on-line tool (which use certain factors such as GC content) to select the best target site within your DNA of interest. We also recommend purification of the dsDNA target and verifying the integrity of the sgRNA on a denaturing gel. If sgRNA efficiency is low, increasing the molar ratio of sgRNA:Sau Cas9:target DNA to 10:10:1 or higher may improve DNA cleavage. We also recommend using the supplied NEBuffer™ 3.1 for best results.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
