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Genome Editing

New England Biolabs provides reagents to support a broad variety of CRISPR/Cas9 genome editing approaches. From introduction of Cas9 and single guide RNA (sgRNA) on plasmids, to direct introduction of Cas9 ribonucleoprotein (RNP) and detection of edits using next generation sequencing or enzymatic mutation detection, NEB provides reagents that simplify and shorten genome editing workflows.

EnGen Spy Cas9 HF1: for high-fidelity genome engineering by direct introduction of active nuclease complexes with reduced off-target cleavage
EnGen^® Spy Cas9 NLS, EnGen Lba Cas12a, EnGen Sau Cas9, EnGen Seq1 Cas9: for genome engineering by direct introduction of active nuclease complexes
EnGen^® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA
EnGen^® Mutation Detection Kit: for robust determination of targeting efficiency
EnGen^® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications
EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates

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Genome Editing includes these subcategories:

Mutation Detection Reagents and Kits
Programmable Nucleases
sgRNA Synthesis Reagents and Kits

Protocols for Genome Editing

Application Notes for Genome Editing

Rapid Analysis of Genome Editing Efficiency using PCR Amplicons processed by the Exo-CIP Rapid PCR Cleanup Kit followed by Sanger sequencing

Tools & Resources

Brochures

Genome Editing Brochure

Posters

Kinetic Comparison of Cas9 Homologs Recognizing Diverse PAM Sequences (2018)

Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Videos

Bacterial Adaptive Immunity with CRISPR/Cas9

CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro.
Science in 60 CRISPR/Cas9

Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.

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Brochures

Posters

Videos

Bacterial Adaptive Immunity with CRISPR/Cas9

Science in 60 CRISPR/Cas9

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