E. coli RNA Polymerase, Core Enzyme
E. coli RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω.
- Free of sigma factor and does not recognize any specific bacterial or phage DNA promoters.
- Retains the ability to transcribe RNA from nonspecific initiation sequences.
- Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters.
- The core enzyme has a molecular weight of approximately 400 kDa.
Avoiding RNase Contamination
Product SourceE. coli RNA Polymerase, Core Enzyme is isolated from E. coli strain BL21.
The following reagents are supplied with this product:
Store at (°C) Concentration E.coli RNA Polymerase Reaction Buffer -20 5 X
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- RNA Synthesis
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