ProtoScript® II Reverse Transcriptase
- Recombinant M-MuLV reverse transcriptase
- Reduced RNase H activity
- Increased thermostability
Avoiding RNase Contamination
ProtoScript® II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
Product SourceThe gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H–) is expressed in E. coli and purified to near homogeneity.
The following reagents are supplied with this product:
Store at (°C) Concentration ProtoScript® II Reverse Transcriptase Reaction Buffer -20 5 X DTT (100mM) -20 10 X
- Product Categories:
- RT-PCR Products,
- Reverse Transcriptases & RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.