ProtoScript® II Reverse Transcriptase
- Recombinant M-MuLV reverse transcriptase
- Reduced RNase H activity
- Increased thermostability
Avoiding RNase Contamination
ProtoScript® II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
Product SourceThe gene encoding a mutant M-MuLV Reverse Transcriptase (RNase H–) is expressed in E. coli and purified to near homogeneity.
The following reagents are supplied with this product:
Store at (°C) Concentration ProtoScript® II Reverse Transcriptase Reaction Buffer -20 5 X DTT (100mM) -20 10 X
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- Reverse Transcriptases & RT-PCR,
- cDNA Synthesis & Reverse Transcriptases
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