RT-PCR
LunaScript Multiplex One-Step RT-PCR Kit
The LunaScript Multiplex One-Step RT-PCR Kit (NEB #E1555) offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. The 5X reaction mix contains a reaction buffer supplemented with dNTPs and is optimized for robust multiple target detection in a simple workflow. The 25X enzyme mix features Luna® WarmStart® Reverse Transcriptase and Q5® Hot Start High-Fidelity DNA Polymerase.
OneTaq® One-Step RT-PCR Kit
The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase.
OneTaq® RT-PCR Kit
The OneTaq® RT-PCR Kit (NEB #E5310) combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions.
LunaScript®, Warmstart®, Q5®, ProtoScript®, LongAmp®, and OneTaq® are registered trademarks of New England Biolabs, Inc.
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Choose Type:
- First Strand cDNA Synthesis Protocols (E6300)
- First strand cDNA synthesis OneTaq® RT-PCR Kit
- PCR Amplification with OneTaq® RT-PCR Kit
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0277)
- First Strand cDNA Synthesis Protocols (E6560)
- One-Step RT-PCR Protocols (E5315)
- Reaction Conditions (E5315)
- Standard PCR Protocol (E5315)
- Typical cDNA Synthesis Protocol with WarmStart RTx (NEB #M0380)
- Typical RT-LAMP Protocol
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0277)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0277)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0253)
- First Strand cDNA Synthesis (No-RT Negative Control Reaction) (NEB #M0368)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Quick Protocol) (NEB #M0368)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0253)
- First Strand cDNA Synthesis (Standard Protocol) (NEB #M0368)
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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