T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase (1). As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
Product SourceAn E. coli strain containing a recombinant gene encoding T3 DNA Ligase.
The following reagents are supplied with this product:
Store at (°C) Concentration T3 DNA Ligase Reaction Buffer 2X
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