HomeFAQsDoes T3 DNA Ligase have a higher tolerance to salt than T4 DNA Ligase?
FAQ: Does T3 DNA Ligase have a higher tolerance to salt than T4 DNA Ligase?
Yes. T3 DNA Ligase can tolerate salt concentrations up to 250-300 mM in the reaction. Please note in the presence of PEG, DNA in a high salt solution will precipitate, inhibiting ligation. If you want to ligate DNA in the presence of salt over 100 mM, we recommend the use of 1X T4 DNA Ligase Buffer.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.