FAQ: Does T3 DNA Ligase have a higher tolerance to salt than T4 DNA Ligase?

Yes. T3 DNA Ligase can tolerate salt concentrations up to 250-300 mM in the reaction. Please note in the presence of PEG, DNA in a high salt solution will precipitate, inhibiting ligation. If you want to ligate DNA in the presence of salt over 100 mM, we recommend the use of 1X T4 DNA Ligase Buffer.