- Single-cell gel electrophoresis (Comet Assay)
- Alkaline elution
- Alkaline unwinding
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Endonuclease IV can act on a variety of oxidative damage in DNA (1). The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.
Product SourceAn E.coli strain which carries the cloned Endo IV gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 3 -20 10 X
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