- DNA AP endonuclease
- Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini
- Also has 3'-diesterase activity which can remove 3' phosphate, 3'-α, β-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups
Endonuclease IV can act on a variety of oxidative damage in DNA (1). The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5´ to the lesion leaving a hydroxyl group at the 3´ terminus and a deoxyribose 5´-phosphate at the 5´ terminus. The enzyme also has a 3´ -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5-phosphate and phosphate from the 3´ end of DNA.
Product SourceAn E.coli strain which carries the cloned Endo IV gene.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 3 -20 10 X
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