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  • NEBuffer 2

    Description

    New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 (NEB #B7201), NEBuffer 3.1 (NEB #B7203) and CutSmart Buffer (NEB #B7204) are available.

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    50mM NaCl
    10mM Tris-HCl
    10mM MgCl2
    1mM DTT
    pH 7.9@25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Conductivity (Buffer/Solution) :
      The conductivity is measured and meets specification.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Functional Test (Restriction Digest, Buffer):
      The buffer is tested for function by performing a restriction enzyme activity assay.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • pH (Buffer/Solution):
      The pH is measured and meets specification.
    • RNase Activity (Buffer):
      The buffer is tested in a reaction containing a RNA substrate. After incubation there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.