NEBuffer 3

Description

New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. The NEBuffer minus BSA formulation is included in this buffer pack for those enzymes requiring NEBuffers without BSA. For the BSA containing formulation, Buffer packs NEBuffer 1.1 (NEB #B7201), NEBuffer 2.1 (NEB #B7201), NEBuffer 3.1 (NEB #B7203) and CutSmart Buffer (NEB #B7204) are available.

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

100mM NaCl
50mM Tris-HCl
10mM MgCl2
1mM DTT
pH 7.9@25°C

FAQs

  1. Why do I see additional DNA bands on my gel after a restriction digest?
  2. Why is my Restriction Enzyme not cutting DNA?
  3. Why do I see a DNA smear on an agarose gel after a restriction digest?
  4. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Conductivity (Buffer/Solution) :
    The conductivity is measured and meets specification.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Functional Test (Restriction Digest, Buffer):
    The buffer is tested for function by performing a restriction enzyme activity assay.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
  • pH (Buffer/Solution):
    The pH is measured and meets specification.
  • RNase Activity (Buffer):
    The buffer is tested in a reaction containing a RNA substrate. After incubation there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.