CutSmart® Buffer

Description

New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

Over 205 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

50mM Potassium Acetate
20mM Tris-acetate
10mM Magnesium Acetate
100μg/ml BSA
pH 7.9@25°C

Notes

  1. The pH of 10X CutSmart Buffer is between pH 7.8 and 8.0.

FAQs

  1. What is supplied with the CutSmart™ Buffer pack?
  2. How should the NEBuffer™ be used?
  3. The buffer arrived thawed. Are the buffer and enzyme still active?
  4. I currently have an old tube of Restriction Enzyme - is it still active in the new buffer?
  5. You have replaced NEBuffer™ 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?
  6. Why did you add BSA into all the restriction enzyme reaction buffers?
  7. Why did you remove DTT from your restriction enzyme buffers?
  8. What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
  9. I don’t want to use BSA in my buffer. What are my options? 
  10. Which restriction enzymes are used in GoldenBraid Assembly?
  11. Why do I see a DNA smear on an agarose gel after a restriction digest?
  12. Why is my Restriction Enzyme not cutting DNA?
  13. Why do I see additional DNA bands on my gel after a restriction digest?
  14. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Functional Test (Restriction Digest, Buffer):
    The buffer is tested for function by performing a restriction enzyme activity assay.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
  • RNase Activity (Extended Digestion):
    The product is tested in a reaction containing a RNA substrate. After incubation for 16 hours greater than 90% of the substrate RNA remains intact as determined by gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.