New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
Over 210 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.
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