This product has been replaced by rCutSmart Buffer (NEB #B6004).
New England Biolabs provides a colorcoded
10X NEBuffer with each restriction endonuclease
to ensure optimal (100%) activity. Most of our
enzymes are supplied with one of four standard
NEBuffers. Occasionally, an enzyme has specific buffer
requirements not met by one of the four standard
NEBuffers, in which case the enzyme is supplied with
its own unique NEBuffer.
Over 215 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions. Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.
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