Storage Temperature

-20°C

1X Buffer Components

50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9@25°C

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. The pH of 10X CutSmart Buffer is between pH 7.8 and 8.0.

1. What is supplied with the CutSmart™ Buffer pack?
2. How should the NEBuffer be used?
3. The buffer arrived thawed. Are the buffer and enzyme still active?
4. I currently have an old tube of Restriction Enzyme - is it still active in the new buffer?
5. You have replaced NEBuffer 1 with NEbuffer 1.1, NEBuffer 2 with NEBuffer 2.1, and NEBuffer 3 with 3.1. Where is NEBuffer 4.1?
6. Why did you add BSA into all the restriction enzyme reaction buffers?
7. Why did you remove DTT from your restriction enzyme buffers?
8. What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
9. I don’t want to use BSA in my buffer. What are my options? 
10. Which restriction enzymes are used in GoldenBraid Assembly?
11. Why do I see a DNA smear on an agarose gel after a restriction digest?
12. Why is my Restriction Enzyme not cutting DNA?
13. Why do I see additional DNA bands on my gel after a restriction digest?
14. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

• Buffer and Diluent Formulation Table

• Activity of DNA Modifying Enzymes in CutSmart® Buffer

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• B7204S_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• B7204S_v1_2971802
• B7204S_v1_2981802
• B7204S_v1_2991802
• B7204S_v1_3001802
• B7204S_v1_3011802
• B7204S_v1_3031803
• B7204S_v1_3041803

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• CutSmart® Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Trademarks

CUTSMART™ is a trademark of New England Biolabs, Inc.