Uracil Glycosylase Inhibitor (UGI)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0281S     -20       Uracil Glycosylase Inhibitor (UGI) M0281SVIAL -20 1 x 0.1 ml 2,000 units/ml   UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X
M0281L     -20       Uracil Glycosylase Inhibitor (UGI) M0281LVIAL -20 1 x 0.5 ml 2,000 units/ml   UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Uracil glycosylase inhibitor (UGI) inhibits uracil-DNA glycosylase (UDG). Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.

Materials Sold Separately

• UDG Reaction Buffer

1. Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.
2. Works only on E. coli UDG (NEB #M0280).  
3. Use equal or more units of UGI than units of UDG in the original reaction.
4. Active in NEBuffers 1-4.  (NEB #B7001, NEB #B7002, NEB #B7003, NEB #B7004)
5. UGI is extremely thermostable, retaining > 95% activity after boiling for 10 minutes.

1. Lindahl, T. et al. (1977). J. Biol.Chem.. 252, 3286-3294.
2. Wang, Z., et al. (1991). Gene. 99, 31-37.
3. Bennett, S. E. and Mosbaugh, D. W. (1992). J. Biol. Chem.. 267, 22512-22521.

• DNA Damage and PreCR

1. What is the nature of the Uracil Glycosylase Inhibitor?
2. How does UGI inhibit UDG?
3. Does UGI inhibit the activity of all UDGs?
4. What is the molecular weight of UGI?
5. Why is it necessary to use UGI in a reaction?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0281S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0281-2000_v0_10007122
• M0281L_v1_10021356
• M0281S_v1_10021354
• M0281L_v1_10038985
• M0281S_v1_10038983
• M0281L_v1_10052633
• M0281S_v1_10055791
• M0281L_v1_10060976
• M0281S_v1_10068470
• M0281S_v1_10087617
• M0281L_v1_10099478
• M0281S_v1_10098880
• M0281L_v1_10104599
• M0281L_v1_10127904
• M0281S_v1_10127902
• M0281L_v1_10154009
• M0281S_v1_10164155
• M0281S_v1_10170238
• M0281L_v1_10182780

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Uracil Glycosylase Inhibitor (UGI)
• UDG Reaction Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.