E. coli Poly (A) Polymerase (M0276)
Vortex tube gently before use.
Poly(A) Polymerase can be inactivated by adding EDTA at a final concentration of 10 mM to the reaction.
Poly (A) Polymerase is compatible with the M-MuLV reverse transcriptase buffer. If the intention is to carry out the polyadenylation step followed by reverse transcription, we recommend to set-up these reactions in two sequential steps. The polyadenylation reaction can be done first using the reverse transcriptase buffer. This can be followed by addition of the components necessary for reverse transcription.
We strongly recommend wearing gloves, using nuclease-free tubes and reagents, and thoroughly cleaning pipettes and bench surfaces to avoid RNase contamination.