T4 Polynucleotide Kinase (3' phosphatase minus)
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- 5' phosphorylation of 3' phosphorylated mononucleotide to generate a substrate (pNp) that can be added to the 3' end of DNA or RNA
- 5' end labeling of 3' phosphorylated oligos
DNA Blunting Tutorial
The Mechanism of DNA Phosphorylation
T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3´-monophosphates (1-5). This modified version exhibits full kinase activity with no 3´ phosphatase activity (6,7).
Product SourceAn E. coli strain that carries a plasmid encoding the modified T4 Polynucleotide Kinase gene.
The following reagents are supplied with this product:
Store at (°C) Concentration T4 Polynucleotide Kinase Reaction Buffer -20 10 X
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