T4 Polynucleotide Kinase (3' phosphatase minus)

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  • 5' phosphorylation of DNA/RNA for subsequent ligation
  • End labeling DNA or RNA for probes and DNA sequencing
  • 5' phosphorylation of 3' phosphorylated mononucleotide to generate a substrate (pNp) that can be added to the 3' end of DNA or RNA
  • 5' end labeling of 3' phosphorylated oligos

Ordering Information

  • 10,000 units/ml
    200 units
  • 10,000 units/ml
    1,000 units
  • Product Information
    T4 Polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3´-monophosphates (1-5). This modified version exhibits full kinase activity with no 3´ phosphatase activity (6,7).

    Product Source

    An E. coli strain that carries a plasmid encoding the modified T4 Polynucleotide Kinase gene.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    T4 Polynucleotide Kinase Reaction Buffer -20 10 X
    Product Categories:
    RNA Modification
    Phosphorylation (Kinase)
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