
Kinases
Choose Product:
Choose Type:
- DNA Ligase Selection Chart
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- End-Modification Tips
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
Selection Tools
Troubleshooting Guides
Usage Guidelines
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
T4 Polynucleotide Kinase (NEB #M0201) can be used to 5' end label DNA and RNA because it catalyzes the transfer and exchange of phosphate from ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates (1).
T4 Polynucleotide Kinase (3' phosphatase minus) (NEB #M0236) is a modified version that exhibits full kinase activity with no 3´ phosphatase activity (2,3).
NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right kinase, choose "End Modification" on the tool to start.
References
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.