DNA Polymerase I, Large (Klenow) Fragment
DNA Polymerase I, Large (Klenow) fragment was originally derived as a proteolytic product of E.coli DNA polymerase that retains polymerase and 3’ —> 5’ exonuclease activity
- Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
- Lacks 5’ —> 3’ exonuclease activity
- Generates probes using random primers
- Second strand cDNA synthesis
DNA Blunting Tutorial
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3'→ 5' exonuclease activity, but has lost 5'→ 3' exonuclease activity (1). Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Product SourceAn E. coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 2 -20 10X
- Product Categories:
- DNA Manipulation Products
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.