New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

NEBExpress® MBP Fusion and Purification System

This product is a direct replacement for NEB #E8200, pMAL™ Protein Fusion and Purification System

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
  • Gentle elution with maltose; no detergents or harsh denaturants required
 
2019-20_Catalog_Promo_0519
Catalog # Concentration Size List Price Your Price Quantity
E8201S Not Applicable 1 set $677.00
Please enter a quantity for at least one size
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