The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|pMAL-c6T Vector||-20||200 µg/ml|
|TEV Protease||-20||10,000 units/ml|
|TEV Protease Reaction Buffer||-20||10 X|
|Anti-MBP Monoclonal Antibody||-20|
|MBP6 Protein||-20||40 µg/ml|
|MBP6-TEV-Paramyosin ΔSal||-20||5 mg/ml|
|E. coli ER2523 (NEB Express) (Glycerol Stock)||-20|
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