Amylose Resin

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E8021S     4       Amylose Resin E8021SVIAL 4 1 x 15 ml Not Applicable
E8021L     4       Amylose Resin E8021LVIAL 4 1 x 100 ml Not Applicable

1. Store at 4°C. After use, resin should be stored incolumn buffer plus 0.02% sodium azide or 20% ethanol.
2. Amylose Resin column should be washed with 5 volumes ofcolumn buffer before each use.
3. For optimum performance, load crude extract at < 60cm/hour.
4. When regenerating the column at 4°C, please note that0.1% SDS can precipitate at that temperature. It is therefore recommended thatthe SDS solution be stored at room temperature until needed. The resin may begenerated up to five times.
5. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8200) from www.neb.com.
6. Regeneration: The packed resin may beregenerated by the following wash sequence: Water 3 - column volumes, 0.1% SDS -3 column volumes, Water - 1 column volume, Column Buffer - 5 columnvolumes.
   

• Purification Beads, Columns and Resins

1. Much of my fusion protein flows through the amylose column. Is there anything I can do to improve my fusion’s affinity for the amylose column?
2. How many times can I use the amylose column?
3. What is known about binding in the presence of nonionic detergents?
4. Can I substitute a different buffer and/or salt concentration in the Column Buffer?
5. I see my intact fusion protein by SDS-PAGE when I run cells boiled in Sample Buffer, but when I check the crude extract the fusion is degraded.
6. When I run my purified fusion protein on SDS-PAGE, why do I see multiple bands instead of a single band of the expected MW?
7. Can I perform a batch purification using the amylose resin?
8. Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
9. Is the amylose resin damaged by storage at -20°C?
10. What gravity column do you recommend?
11. Can amylose resin be cleaned, stored, and used again?

• Protein Expression & Purification Brochure
• Purification Beads, Columns and Resins Brochure

• Over 40 years in protein expression and purification &ndash; a historical perspective

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E8021S_L_v1
• E8021S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E8021S_L_v1_0221710
• E8021L_v1_10012601
• E8021S_v1_10012792
• E8021L_v1_10015076
• E8021S_v1_10015170
• E8021S_v1_10022013
• E8021L_v1_10022014
• E8021S_v1_10025025
• E8021L_v1_10027728
• E8021L_v1_10027726
• E8021S_v1_10030168
• E8021L_v1_10031638
• E8021S_v1_10031639
• E8021S_v1_10040397
• E8021L_v1_10041603
• E8021S_v1_10045741
• E8021S_v1_10047454
• E8021L_v1_10045743
• E8021L_v1_10049208
• E8021S_v1_10049209
• E8021L_v1_10052793
• E8021S_v1_10053919
• E8021L_v1_10054599
• E8021S_v1_10054600
• E8021L_v1_10058504
• E8021S_v1_10061013
• E8021L_v1_10061015
• E8021S_v1_10064287
• E8021S_v1_10066736
• E8021L_v1_10065936
• E8021S_v1_10069982
• E8021S_v1_10072114
• E8021L_v1_10072113
• E8021L_v1_10077451
• E8021S_v1_10077452
• E8021L_v1_10085921
• E8021S_v1_10083353
• E8021L_v1_10087455
• E8021S_v1_10087456
• E8021L_v1_10091815
• E8021S_v1_10092420
• E8021S_v1_10097700
• E8021L_v1_10097167
• E8021S_v1_10102232
• E8021L_v1_10105390
• E8021S_v1_10105391
• E8021S_v1_10111314
• E8021L_v1_10111313
• E8021S_v1_10115983
• E8021L_v1_10115982
• E8021S_v1_10121350
• E8021S_v1_10128178
• E8021L_v2_10126407
• E8021L_v2_10130297
• E8021S_v2_10130296
• E8021L_v2_10140909
• E8021S_v2_10140918
• E8021L_v2_10140919
• E8021L_v2_10146247
• E8021S_v2_10146250
• E8021L_v2_10151466
• E8021S_v2_10155100
• E8021L_v2_10155101
• E8021L_v2_10160089
• E8021S_v2_10162187
• E8021S_v2_10163108
• E8021L_v2_10168535
• E8021S_v2_10168536
• E8021L_v2_10169879
• E8021S_v2_10169880
• E8021S_v2_10176035
• E8021L_v2_10176034
• E8021S_v2_10178545
• E8021L_v2_10181436
• E8021S_v2_10184625
• E8021L_v2_10190415
• E8021S_v2_10197132
• E8021L_v2_10197133
• E8021S_v2_10198027
• E8021L_v2_10203699
• E8021L_v2_10206255
• E8021S_v2_10207558
• E8021L_v2_10212242
• E8021S_L_v1_0221801
• E8021S_L_v1_0231804
• E8021L_v2_10221723
• E8021S_v2_10219382
• E8021S_v2_10221724
• E8021L_v2_10227033
• E8021S_v2_10229423
• E8021L_v2_10238367
• E8021S_v2_10238368
• E8021L_v2_10249337
• E8021S_v2_10251152
• E8021L_v2_10254240

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Amylose Resin

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.