An ideal library will represent completely and proportionally the sequence of the input RNA of interest. When library preparation is inefficient or when input amounts for a library are very low, there is a risk that the resulting library will lack this diversity, and that some sequences will be over- or under-represented. Correlation analysis shows that NEBNext Ultra II RNA libraries retain complexity even at low input amounts.
Libraries show superior complexity at a range of input amounts
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at email@example.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.