An ideal library will represent completely and proportionally the sequence of the input RNA of interest. When library preparation is inefficient or when input amounts for a library are very low, there is a risk that the resulting library will lack this diversity, and that some sequences will be over- or under-represented. Correlation analysis shows that NEBNext Ultra II RNA libraries retain complexity even at low input amounts.
Libraries show superior complexity at a range of input amounts
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NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.