NEBNext® Ultra™ Ligation Module

Description







 
The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

The NEBNext Ultra™ Ligation Module is Designed for Use with the Following Products:
NEBNext Ultra™ End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335#E7500, #E7710, #E7730, #E6609, #E7600)
NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a DNA library on the Illumina sequencing platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext Ligation Enhancer-20
Blunt/TA Ligase Master Mix-20

Properties and Usage

Materials Required but not Supplied

  • NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
  • NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500 or #E7600)
  • NEBNext Q5 Hot Start HiFi PCR Master Mix (NEB #M0543)
  • NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)

Storage Temperature

-20°C

FAQs

  1. What is the difference between the NEBNext Ultra Ligation Module and the NEBNext Quick Ligation Module?
  2. How do I obtain the NEBNext Adaptor and USER enzyme for use with the NEBNext Ultra Ligation Module?

Protocols

  1. Please see manual (NEB #E7445) for protocols

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Interactive Tools

Application Notes

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.