NEBNext® Ultra™ End Repair/dA-Tailing Module
However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 μg), and a NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) is available.
The NEBNext® Ultra™ End Repair/dA-Tailing Module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, 3’ dA-tailed ends. The module is optimized for use with NEBNext Ultra Ligation Module (NEB #E7445), and is part of the original Ultra workflow, which enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext End Prep Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X
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