NEBNext® Ultra™ End Repair/dA-Tailing Module

Description







 
The NEBNext ® End Prep module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, dA-tailed ends.

The NEBNext Ultra™ End Repair/dA-Tailing Module Is Designed For Use with the Following:
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335 or #E7500)
NEBNext Ultra™ Ligation Module (NEB #E7445)
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)

Functional Validation

Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a .

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext End Prep Enzyme Mix-20
NEBNext End Repair Reaction Buffer-2010X

Properties and Usage

Storage Temperature

-20°C

FAQs

  1. There are 2 NEBNext Ligation modules, the NEBNext Ultra Ligation Module (#E7445) and the NEBNext Quick Ligation Module(#E6056). Which module should be used in conjuction with the NEBNext Ultra End Repair/dA-Tailing Module?
  2. How do I clean up the reaction once complete?

Protocols

  1. NEBNext End Prep (E7442)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Application Notes

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.