NEBNext® Ultra™ End Repair/dA-Tailing Module
However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 μg), and a NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) is available.
The NEBNext® Ultra™ End Repair/dA-Tailing Module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, 3’ dA-tailed ends. The module is optimized for use with NEBNext Ultra Ligation Module (NEB #E7445), and is part of the original Ultra workflow, which enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext End Prep Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10X
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