NEBNext® Ultra™ End Repair/dA-Tailing Module

1. NEBNext End Prep (E7442)

• Low input ChIPSeq using the NEBNext Ultra Library Prep Kit for Illumina E7442

1. There are 2 NEBNext Ligation modules, the NEBNext Ultra Ligation Module (#E7445) and the NEBNext Quick Ligation Module(#E6056). Which module should be used in conjuction with the NEBNext Ultra End Repair/dA-Tailing Module?
2. How do I clean up the reaction once complete?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7442S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7442L_v0_10009544
• E7442L_v1_10013684
• E7442S_v1_10013847
• E7442S_v1_10015100
• E7442L_v1_10009544
• E7442L_v1_10019223
• E7442L_v1_10020455
• E7442S_v1_10026729
• E7442L_v1_10029005
• E7442S_v1_10034749
• E7442L_v1_10036488
• E7442S_v1_10040263
• E7442S_v1_10020452
• E7442L_v1_10048325
• E7442L_v1_10051555
• E7442S_v1_10054379
• E7442L_v1_10054999
• E7442L_v1_10033347
• E7442S_v1_10060543
• E7442S_v1_10064724
• E7442L_v1_10065505
• E7442L_v1_10072285
• E7442S_v1_10044400
• E7442S_v1_10077046
• E7442L_v1_10080301
• E7442L_v1_10059737
• E7442S_v1_10083189
• E7442L_v1_10090853
• E7442S_v1_10094833
• E7442L_v1_10101443

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext End Prep Enzyme Mix
• NEBNext End Repair Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.