NEBNext® Ultra™ End Repair/dA-Tailing Module
However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 μg), and a NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) is available.
Ordering Information
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Product Information
The NEBNext® Ultra™ End Repair/dA-Tailing Module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, 3’ dA-tailed ends. The module is optimized for use with NEBNext Ultra Ligation Module (NEB #E7445), and is part of the original Ultra workflow, which enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component pageReagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext End Prep Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X - Product Categories:
- RNA Library Prep
- Applications:
- DNA for Illumina®,
- Illumina® Library Preparation
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FAQs & Tech Tips
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Protocols & Manuals
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Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].)
- Protocols
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Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
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Manuals
- Other Tools & Resources
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Quality & Safety
- Quality Control Assays
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
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Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
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Legal Information
manualE7442
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