The NEBNext ® End Prep module is optimized to convert 5 ng–1 μg of fragmented
DNA to repaired DNA having 5' phosphorylated, dA-tailed ends.
The NEBNext Ultra™ End Repair/dA-Tailing
Module Is Designed For Use with the Following:
NEBNext Singleplex or Multiplex Oligos for Illumina® (NEB #E7350, #E7335 or #E7500)
NEBNext Ultra™ Ligation Module (NEB #E7445)
NEBNext High-Fidelity 2X PCR Master Mix (NEB #M0541)
Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a .
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
|Store at (°C)||Concentration
|NEBNext End Prep Enzyme Mix||-20||
|NEBNext End Repair Reaction Buffer||-20||10X