NEBNext® High-Fidelity 2X PCR Master Mix

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NEBNext® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.

However, the NEBNext Ultra II Q5 Master Mix (NEB #M0544) is now available, and this more recent formulation is a hot start and also further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

Ordering Information

M0541S_pdp_800X450
  • 2 X
    50 reactions
    $90.00
  • 2 X
    250 reactions
    $360.00
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