NEBNext® High-Fidelity 2X PCR Master Mix
icon_cloned icon_recomb icon_HI_no

New

NEBNext® High-Fidelity 2X PCR Master Mix is the original non-hot start NEBNext formulation of Q5® High-Fidelity DNA Polymerase. This formulation is also included as a component in the original NEBNext standard workflow library prep kits for Illumina, and in NEBNext library prep kits for Ion Torrent.

However, the NEBNext Ultra II Q5 Master Mix (NEB #M0544) is now available, and this more recent formulation is a hot start and also further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions. The NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size List Price Your Price Quantity
M0541S 2X 50 reactions $90.00
M0541L 2X 250 reactions $360.00
Please enter a quantity for at least one size
Loading Spinner