Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Q5® High-Fidelity DNA Polymerase is an ultra high-fidelity, thermostable DNA
polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing
Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase
is ideal for cloning and can be used for long or difficult amplicons. The Q5
High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a
convenient master mix format. The mix contains dNTPs, Mg++ and a proprietary
broad-use buffer requiring only the addition of primers and DNA template for
robust amplification, regardless of GC content. When used at the recommended
1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl2.
Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions (NEB #E0555S) or 200 reactions (NEB #E0555L). For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.