Q5® High-Fidelity DNA Polymerase is an ultra high-fidelity, thermostable DNA
polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing
Sso7d domain to support robust DNA amplification. With an error rate > 100-
fold lower than that of Taq DNA Polymerase and 12-fold lower than that of
Pyrococcus furiosus ( Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase
is ideal for cloning and can be used for long or difficult amplicons. The Q5
High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a
convenient master mix format. The mix contains dNTPs, Mg ++ and a proprietary
broad-use buffer requiring only the addition of primers and DNA template for
robust amplification, regardless of GC content. When used at the recommended
1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl 2.
Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
To determine the optimal annealing temperatures for a given set of primers,
use of the NEB Tm Calculator is highly recommended.
This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions (NEB #E0555S) or 200 reactions (NEB #E0555L). For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.
The following reagents are supplied with this product:
Store at (°C) Concentration
Q5® High-Fidelity 2X Master Mix -20 2X
Nuclease-free Water -20
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb) 4 100 μg/ml
Gel Loading Dye, Blue (6X) 25 6X