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DNA Modifying Enzymes & Cloning Technologies

NEB offers a comprehensive list of tools and reagents for DNA manipulation. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

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DNA Modifying Enzymes and Cloning Technologies
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5-hydroxymethyluridine DNA Kinase

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Immobilized T4 DNA Ligase

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Nucleoside Digestion Mix

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Quick CIP

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Thermolabile Exonuclease I

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Tth Argonaute (TtAgo)

Argonautes

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Tth Argonaute (TtAgo)

DNA Ligases

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9°N™ DNA Ligase

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Blunt/TA Ligase Master Mix

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E. coli DNA Ligase

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ElectroLigase®

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HiFi Taq DNA Ligase

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Hi-T4™ DNA Ligase

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Immobilized T4 DNA Ligase

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Instant Sticky-end Ligase Master Mix

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NEBNext® Quick Ligation Module

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Quick Blunting™ and Quick Ligation™ Kits

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Quick Ligation™ Kit

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Salt-T4® DNA Ligase

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SplintR® Ligase

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StickTogether™ DNA Ligase Buffer

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T3 DNA Ligase

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T4 DNA Ligase

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T4 DNA Ligase Reaction Buffer

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T7 DNA Ligase

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Taq DNA Ligase

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Taq DNA Ligase Reaction Buffer

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Thermostable 5´ App DNA/RNA Ligase

DNA Repair Enzymes and Structure-specific Endonucleases

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Afu Uracil-DNA Glycosylase (UDG)

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Antarctic Thermolabile UDG

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APE 1

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Endonuclease III (Nth)

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Endonuclease IV

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Endonuclease V

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Endonuclease VIII

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EnGen® Mutation Detection Kit

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Fpg

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hAAG

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hSMUG1

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Mismatch Endonuclease I

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PreCR® Repair Mix

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RecA

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RecAf

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T4 PDG (T4 Endonuclease V)

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T7 Endonuclease I

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Thermolabile USER® II Enzyme

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Thermostable FEN1

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Tma Endonuclease III

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Topoisomerase I (E. coli)

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Tth Endonuclease IV

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Uracil Glycosylase Inhibitor (UGI)

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Uracil-DNA Glycosylase (UDG)

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USER® Enzyme

Methyltransferases

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AluI Methyltransferase

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BamHI Methyltransferase

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CpG Methyltransferase (M.SssI)

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dam Methyltransferase

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EcoGII Methyltransferase

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EcoRI Methyltransferase

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GpC Methyltransferase (M.CviPI)

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HaeIII Methyltransferase

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Hhal Methyltransferase

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HpaII Methyltransferase

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Human DNA (cytosine-5) Methyltransferase (Dnmt1)

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MspI Methyltransferase

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TaqI Methyltransferase

Exonucleases and Non-specific Endonucleases

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DNase I (RNase-free)

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DNase I Reaction Buffer

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Exonuclease I (E. coli)

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Exonuclease I Reaction Buffer

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Exonuclease III (E. coli)

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Exonuclease T

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Exonuclease V (RecBCD)

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Exonuclease VII

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Exonuclease VIII, truncated

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Lambda Exonuclease

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Micrococcal Nuclease

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Mismatch Endonuclease I

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Mung Bean Nuclease

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Nuclease BAL-31

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Nuclease P1

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RecJf

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T5 Exonuclease

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T7 Endonuclease I

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T7 Exonuclease

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Thermolabile Exonuclease I

Phosphatases & Sulfurylases

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Antarctic Phosphatase

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Antarctic Phosphatase Reaction Buffer

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Apyrase

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Pyrophosphatase, Inorganic (E. coli)

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Pyrophosphatase, Inorganic (yeast)

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Quick CIP

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Shrimp Alkaline Phosphatase (rSAP)

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Thermostable Inorganic Pyrophosphatase

Recombinases

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Cre Recombinase

ssDNA Binding Proteins

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ET SSB

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RecA

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RecAf

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T4 Gene 32 Protein

Kinases

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T4 Polynucleotide Kinase

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T4 Polynucleotide Kinase (3' phosphatase minus)

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T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

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DNA Polymerase I (E. coli)

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Klenow Fragment (3'→5' exo-)

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Streptavidin

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T4 Polynucleotide Kinase

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Terminal Transferase

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Therminator™ DNA Polymerase

Other Products

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M13KO7 Helper Phage

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TelN Protelomerase

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β-Agarase I

Argonautes
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Tth Argonaute (TtAgo)

DNA Ligases
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9°N™ DNA Ligase

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Blunt/TA Ligase Master Mix

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E. coli DNA Ligase

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ElectroLigase®

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HiFi Taq DNA Ligase

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Hi-T4™ DNA Ligase

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Immobilized T4 DNA Ligase

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Instant Sticky-end Ligase Master Mix

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NEBNext® Quick Ligation Module

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Quick Blunting™ and Quick Ligation™ Kits

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Quick Ligation™ Kit

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Salt-T4® DNA Ligase

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SplintR® Ligase

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StickTogether™ DNA Ligase Buffer

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T3 DNA Ligase

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T4 DNA Ligase

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T4 DNA Ligase Reaction Buffer

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T7 DNA Ligase

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Taq DNA Ligase

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Taq DNA Ligase Reaction Buffer

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Thermostable 5´ App DNA/RNA Ligase

DNA Repair Enzymes and Structure-specific Endonucleases
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Afu Uracil-DNA Glycosylase (UDG)

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Antarctic Thermolabile UDG

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APE 1

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Endonuclease III (Nth)

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Endonuclease IV

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Endonuclease V

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Endonuclease VIII

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EnGen® Mutation Detection Kit

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Fpg

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hAAG

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hSMUG1

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Mismatch Endonuclease I

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PreCR® Repair Mix

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RecA

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RecAf

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T4 PDG (T4 Endonuclease V)

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T7 Endonuclease I

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Thermolabile USER® II Enzyme

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Thermostable FEN1

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Tma Endonuclease III

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Topoisomerase I (E. coli)

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Tth Endonuclease IV

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Uracil Glycosylase Inhibitor (UGI)

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Uracil-DNA Glycosylase (UDG)

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USER® Enzyme

Methyltransferases
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AluI Methyltransferase

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BamHI Methyltransferase

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CpG Methyltransferase (M.SssI)

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dam Methyltransferase

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EcoGII Methyltransferase

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EcoRI Methyltransferase

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GpC Methyltransferase (M.CviPI)

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HaeIII Methyltransferase

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Hhal Methyltransferase

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HpaII Methyltransferase

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Human DNA (cytosine-5) Methyltransferase (Dnmt1)

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MspI Methyltransferase

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TaqI Methyltransferase

Exonucleases and Non-specific Endonucleases
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DNase I (RNase-free)

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DNase I Reaction Buffer

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Exonuclease I (E. coli)

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Exonuclease I Reaction Buffer

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Exonuclease III (E. coli)

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Exonuclease T

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Exonuclease V (RecBCD)

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Exonuclease VII

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Exonuclease VIII, truncated

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Lambda Exonuclease

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Micrococcal Nuclease

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Mismatch Endonuclease I

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Mung Bean Nuclease

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Nuclease BAL-31

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Nuclease P1

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RecJf

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T5 Exonuclease

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T7 Endonuclease I

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T7 Exonuclease

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Thermolabile Exonuclease I

Phosphatases & Sulfurylases
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Antarctic Phosphatase

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Antarctic Phosphatase Reaction Buffer

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Apyrase

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Pyrophosphatase, Inorganic (E. coli)

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Pyrophosphatase, Inorganic (yeast)

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Quick CIP

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Shrimp Alkaline Phosphatase (rSAP)

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Thermostable Inorganic Pyrophosphatase

Recombinases
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Cre Recombinase

ssDNA Binding Proteins
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ET SSB

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RecA

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RecAf

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T4 Gene 32 Protein

Kinases
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T4 Polynucleotide Kinase

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T4 Polynucleotide Kinase (3' phosphatase minus)

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T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling
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DNA Polymerase I (E. coli)

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Klenow Fragment (3'→5' exo-)

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Streptavidin

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T4 Polynucleotide Kinase

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Terminal Transferase

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Therminator™ DNA Polymerase

Other Products
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M13KO7 Helper Phage

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TelN Protelomerase

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β-Agarase I