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DNA Modifying Enzymes

NEB offers a comprehensive list of tools and reagents for DNA manipulation. These include ligases, nucleases, methyltransferases, DNA repair proteins, recombinases and more.

NEBcloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow.

DNA Modifying Enzymes and Cloning Technologies

DNA Ligases

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Order 9°N™ DNA Ligase

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Order Blunt/TA Ligase Master Mix

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Order E. coli DNA Ligase

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Order ElectroLigase®

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Order HiFi Taq DNA Ligase

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Order Instant Sticky-end Ligase Master Mix

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Order NEBNext® Quick Ligation Module

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Order Quick Blunting™ and Quick Ligation™ Kits

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Order Quick Ligation™ Kit

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Order SplintR® Ligase

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Order T3 DNA Ligase

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Order T4 DNA Ligase

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Order T4 DNA Ligase Reaction Buffer

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Order T7 DNA Ligase

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Order Taq DNA Ligase

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Order Taq DNA Ligase Reaction Buffer

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Order Thermostable 5´ AppDNA/RNA Ligase

DNA Repair Proteins

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Order Afu Uracil-DNA Glycosylase (UDG)

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Order Antarctic Thermolabile UDG

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Order APE 1

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Order Endonuclease III (Nth)

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Order Endonuclease IV

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Order Endonuclease V

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Order Endonuclease VIII

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Order EnGen® Mutation Detection Kit

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Order Fpg

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Order hAAG

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Order hSMUG1

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Order PreCR® Repair Mix

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Order RecA

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Order RecAf

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Order T4 PDG (T4 Endonuclease V)

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Order T7 Endonuclease I

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Order Thermolabile USER® II Enzyme

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Order Tma Endonuclease III

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Order Topoisomerase I (E. coli)

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Order Tth Endonuclease IV

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Order Uracil Glycosylase Inhibitor (UGI)

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Order Uracil-DNA Glycosylase (UDG)

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Order USER® Enzyme

Methyltransferases

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Order AluI Methyltransferase

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Order BamHI Methyltransferase

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Order CpG Methyltransferase (M.SssI)

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Order dam Methyltransferase

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Order EcoGII Methyltransferase

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Order EcoRI Methyltransferase

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Order GpC Methyltransferase (M.CviPI)

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Order Haelll Methyltransferase

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Order Hhal Methyltransferase

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Order HpaII Methyltransferase

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Order Human DNA (cytosine-5) Methyltransferase (Dnmt1)

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Order Human PRMT1 Methyltransferase

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Order MspI Methyltransferase

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Order SET7 Methyltransferase

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Order SET8 Methyltransferase

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Order TaqI Methyltransferase

Nucleases

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Order Afu Uracil-DNA Glycosylase (UDG)

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Order Antarctic Thermolabile UDG

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Order APE 1

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Order Cas9 Nuclease, S. pyogenes

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Order DNase I (RNase-free)

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Order DNase I Reaction Buffer

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Order Endonuclease III (Nth)

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Order Endonuclease IV

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Order Endonuclease V

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Order Endonuclease VIII

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Order EnGen® Cas9 NLS, S. pyogenes

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Order EnGen® Mutation Detection Kit

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Order Exonuclease I (E. coli)

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Order Exonuclease I Reaction Buffer

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Order Exonuclease III (E. coli)

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Order Exonuclease T

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Order Exonuclease V (RecBCD)

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Order Exonuclease VII

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Order Exonuclease VIII, truncated

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Order Fpg

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Order hAAG

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Order hSMUG1

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Order Lambda Exonuclease

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Order Micrococcal Nuclease

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Order Mung Bean Nuclease

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Order Nuclease BAL-31

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Order PreCR® Repair Mix

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Order RecAf

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Order RecJf

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Order T4 PDG (T4 Endonuclease V)

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Order T5 Exonuclease

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Order T7 Endonuclease I

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Order T7 Exonuclease

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Order Thermolabile USER® II Enzyme

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Order Thermostable FEN1

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Order Tma Endonuclease III

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Order Tth Endonuclease IV

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Order Uracil Glycosylase Inhibitor (UGI)

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Order Uracil-DNA Glycosylase (UDG)

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Order USER® Enzyme

Phosphatases & Sulfurylases

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Order Alkaline Phosphatase, Calf Intestinal (CIP)

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Order Antarctic Phosphatase

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Order Antarctic Phosphatase Reaction Buffer

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Order Apyrase

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Order ATP Sulfurylase

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Order Pyrophosphatase, Inorganic (E. coli)

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Order Pyrophosphatase, Inorganic (yeast)

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Order Quick Dephosphorylation Kit

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Order Shrimp Alkaline Phosphatase (rSAP)

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Order Thermostable Inorganic Pyrophosphatase

Recombinases

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Order Cre Recombinase

ssDNA Binding Proteins

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Order ET SSB

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Order RecA

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Order RecAf

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Order T4 Gene 32 Protein

Kinases

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Order T4 Polynucleotide Kinase

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Order T4 Polynucleotide Kinase (3' phosphatase minus)

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Order T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

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Order DNA Polymerase I (E. coli)

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Order Klenow Fragment (3'→5' exo-)

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Order Streptavidin

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Order T4 Polynucleotide Kinase

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Order Terminal Transferase

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Order Therminator™ DNA Polymerase

Other Products

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Order M13KO7 Helper Phage

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Order TelN Protelomerase

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Order β-Agarase I

DNA Ligases

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Order 9°N™ DNA Ligase

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Order Blunt/TA Ligase Master Mix

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Order E. coli DNA Ligase

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Order ElectroLigase®

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Order HiFi Taq DNA Ligase

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Order Instant Sticky-end Ligase Master Mix

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Order NEBNext® Quick Ligation Module

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Order Quick Blunting™ and Quick Ligation™ Kits

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Order Quick Ligation™ Kit

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Order SplintR® Ligase

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Order T3 DNA Ligase

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Order T4 DNA Ligase

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Order T4 DNA Ligase Reaction Buffer

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Order T7 DNA Ligase

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Order Taq DNA Ligase

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Order Taq DNA Ligase Reaction Buffer

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Order Thermostable 5´ AppDNA/RNA Ligase

DNA Repair Proteins

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Order Afu Uracil-DNA Glycosylase (UDG)

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Order Antarctic Thermolabile UDG

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Order APE 1

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Order Endonuclease III (Nth)

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Order Endonuclease IV

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Order Endonuclease V

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Order Endonuclease VIII

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Order EnGen® Mutation Detection Kit

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Order Fpg

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Order hAAG

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Order hSMUG1

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Order PreCR® Repair Mix

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Order RecA

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Order RecAf

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Order T4 PDG (T4 Endonuclease V)

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Order T7 Endonuclease I

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Order Thermolabile USER® II Enzyme

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Order Tma Endonuclease III

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Order Topoisomerase I (E. coli)

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Order Tth Endonuclease IV

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Order Uracil Glycosylase Inhibitor (UGI)

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Order Uracil-DNA Glycosylase (UDG)

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Order USER® Enzyme

Methyltransferases

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Order AluI Methyltransferase

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Order BamHI Methyltransferase

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Order CpG Methyltransferase (M.SssI)

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Order dam Methyltransferase

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Order EcoGII Methyltransferase

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Order EcoRI Methyltransferase

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Order GpC Methyltransferase (M.CviPI)

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Order Haelll Methyltransferase

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Order Hhal Methyltransferase

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Order HpaII Methyltransferase

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Order Human DNA (cytosine-5) Methyltransferase (Dnmt1)

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Order Human PRMT1 Methyltransferase

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Order MspI Methyltransferase

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Order SET7 Methyltransferase

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Order SET8 Methyltransferase

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Order TaqI Methyltransferase

Nucleases

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Order Afu Uracil-DNA Glycosylase (UDG)

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Order Antarctic Thermolabile UDG

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Order APE 1

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Order Cas9 Nuclease, S. pyogenes

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Order DNase I (RNase-free)

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Order DNase I Reaction Buffer

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Order Endonuclease III (Nth)

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Order Endonuclease IV

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Order Endonuclease V

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Order Endonuclease VIII

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Order EnGen® Cas9 NLS, S. pyogenes

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Order EnGen® Mutation Detection Kit

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Order Exonuclease I (E. coli)

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Order Exonuclease I Reaction Buffer

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Order Exonuclease III (E. coli)

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Order Exonuclease T

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Order Exonuclease V (RecBCD)

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Order Exonuclease VII

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Order Exonuclease VIII, truncated

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Order Fpg

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Order hAAG

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Order hSMUG1

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Order Lambda Exonuclease

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Order Micrococcal Nuclease

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Order Mung Bean Nuclease

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Order Nuclease BAL-31

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Order PreCR® Repair Mix

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Order RecAf

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Order RecJf

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Order T4 PDG (T4 Endonuclease V)

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Order T5 Exonuclease

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Order T7 Endonuclease I

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Order T7 Exonuclease

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Order Thermolabile USER® II Enzyme

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Order Thermostable FEN1

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Order Tma Endonuclease III

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Order Tth Endonuclease IV

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Order Uracil Glycosylase Inhibitor (UGI)

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Order Uracil-DNA Glycosylase (UDG)

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Order USER® Enzyme

Phosphatases & Sulfurylases

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Order Alkaline Phosphatase, Calf Intestinal (CIP)

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Order Antarctic Phosphatase

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Order Antarctic Phosphatase Reaction Buffer

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Order Apyrase

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Order ATP Sulfurylase

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Order Pyrophosphatase, Inorganic (E. coli)

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Order Pyrophosphatase, Inorganic (yeast)

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Order Quick Dephosphorylation Kit

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Order Shrimp Alkaline Phosphatase (rSAP)

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Order Thermostable Inorganic Pyrophosphatase

Recombinases

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Order Cre Recombinase

ssDNA Binding Proteins

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Order ET SSB

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Order RecA

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Order RecAf

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Order T4 Gene 32 Protein

Kinases

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Order T4 Polynucleotide Kinase

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Order T4 Polynucleotide Kinase (3' phosphatase minus)

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Order T4 Polynucleotide Kinase Reaction Buffer

DNA Labeling

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Order DNA Polymerase I (E. coli)

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Order Klenow Fragment (3'→5' exo-)

Close

Order Streptavidin

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Order T4 Polynucleotide Kinase

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Order Terminal Transferase

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Order Therminator™ DNA Polymerase

Other Products

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Order M13KO7 Helper Phage

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Order TelN Protelomerase

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Order β-Agarase I