Topoisomerase I (E. coli)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
NEB # Stored at (°C) M0301S -20 Component Name Component # Stored at (°C) Amount Concentration Topoisomerase I (E. coli) M0301SVIAL -20 1 x 0.02 ml 5,000 units/ml rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
NEB # Stored at (°C) M0301L -20 Component Name Component # Stored at (°C) Amount Concentration Topoisomerase I (E. coli) M0301LVIAL -20 1 x 0.1 ml 5,000 units/ml rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

1. Liu, L.F., Depew, R.E. and Wang, J.C. (1976). J. Mol. Biol.. 106, 439-452.
2. Kirkegaard, K. and Wang, J.C. (1978). Nucl. Acids Res.. 5, 3811-3820.

1. Protocol for Topoisomerase I (E. coli) (M0301)

• DNA Damage and PreCR

1. Does Topoisomerase I relax all supercoiled DNA to the same degree or are the products an equilibrium of various conformations?
2. If the DNA resulting from a Topoisomerase I reaction is run in an ethidium bromide gel and there seems to be no evidence of enzyme activity, what is the most probable explanation?
3. What is the molecular weight of Topoisomerase I?
4. Can Topoisomerase I relax both positively and negatively supercoiled DNA?
5. Does E. coli Toposiomerase I have the same properties as the Vaccinia Topoisomerase I?
6. Will Topoisomerase I produce single strand-breaks in a plasmid?
7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
8. What is the difference between DNA Gyrase (E. coli) (NEB #M7636), Topoisomerase I (E. coli) (NEB #M0301), and 9°N^™ Reverse Gyrase (NEB #M0200)?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0301S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0301S_L_v1_0081609
• M0301S_L_v1_0081701
• M0301S_L_v1_0081705
• M0301S_L_v1_0081708
• M0301S_L_v1_0081803
• M0301S_v1_10018063
• M0301L_v1_10022123
• M0301S_v1_10034985
• M0301L_v1_10034988
• M0301S_v1_10042002
• M0301L_v1_10042004
• M0301S_v1_10051071
• M0301L_v1_10060424
• M0301S_v1_10060425
• M0301L_v1_10077440
• M0301S_v1_10077439
• M0301L_v1_10081888
• M0301S_v1_10081887
• M0301L_v1_10090024
• M0301S_v1_10090025
• M0301S_v1_10092455
• M0301L_v1_10092453
• M0301S_v1_10102185
• M0301L_v1_10102188
• M0301L_v1_10123122
• M0301S_v1_10123121
• M0301S_v1_10134555
• M0301L_v1_10145544
• M0301S_v1_10153791
• M0301L_v1_10153793
• M0301S_v1_10178739
• M0301L_v1_10184023
• M0301S_v1_10192458
• M0301S_v1_10197168
• M0301L_v1_10192459
• M0301L_v1_10218655
• M0301L_v1_10221850
• M0301S_v1_10221851
• M0301L_v1_10234128
• M0301S_v1_10236072
• M0301L_v1_10248322
• M0301S_v1_10260841
• M0301L_v1_10266156
• M0301S_v1_10270559
• M0301L_v1_10288990
• M0301S_v1_10298683
• M0301L_v1_10320069
• M0301S_v1_10323184
• M0301L_v1_10337083
• M0301S_v1_10338290
• M0301S_v1_10349888
• M0301L_v1_10345223

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Topoisomerase I (E. coli)
• rCutSmart™ Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.