Home DNA Modifying Enzymes and Cloning Technologies Uracil Glycosylase Inhibitor (UGI)

Uracil Glycosylase Inhibitor (UGI)
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  • Uracil Glycosylase Inhibitor is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species
  • Remains partially active following heat treatment at 95°C
  • Can be used to prevent subsequent degradation of product DNA

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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M0281S 2,000 units/ml 200 units $88.00 $88.00
M0281L 2,000 units/ml 1,000 units $358.00 $358.00
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  • The Uracil Glycosylase Inhibitor (UGI) of Bacillus subtilis bacteriophage PBS1 is a small protein (9.5 kDa) which inhibits E. coli uracil-DNA glycosylase (UDG) as well as UDG from other species. Inhibition of UDG occurs by reversible protein binding with a 1:1 UGD:UGI stoichiometry. UGI is capable of dissociating UDG-DNA complexes.

    Product Source

    An E. coli strain that carries the cloned UGI gene from Bacillus subtilis bacteriophage PBS1.
    This product is related to the following categories:
    DNA Repair Enzymes and Structure-specific Endonucleases

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration
      • M0281S     -20    
          Uracil Glycosylase Inhibitor (UGI) M0281SVIAL -20 1 x 0.1 ml 2,000 units/ml
          UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X
      • M0281L     -20    
          Uracil Glycosylase Inhibitor (UGI) M0281LVIAL -20 1 x 0.5 ml 2,000 units/ml
          UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X

    • Properties & Usage

      Unit Definition

      One unit of UGI is defined as the amount of protein required to inhibit one unit of E. coli UDG in 1 hour at 37°C in a total reaction volume of 50 µl. One unit of UDG is the amount of enzyme which will catalyze the release of 60 pmol of uracil per minute from double stranded, uracil-containing DNA.

      Reaction Conditions

      1X UDG Reaction Buffer
      Incubate at 37°C

      1X UDG Reaction Buffer
      20 mM Tris-HCl
      1 mM DTT
      1 mM EDTA
      (pH 8 @ 25°C)

      Storage Buffer

      10 mM Tris-HCl
      50 mM KCl
      1 mM DTT
      0.1 mM EDTA
      200 µg/ml BSA
      50% Glycerol
      pH 7.4 @ 25°C

      Heat Inactivation

      70°C for 10 minutes

    • Advantages and Features

      Application Features

      • Uracil glycosylase inhibitor (UGI) inhibits uracil-DNA glycosylase (UDG). Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.

    • Related Products

      Materials Sold Separately

    • Product Notes

      1. Since UDG remains partially active following heat treatment at 95°C, UGI can be used to prevent subsequent degradation of product DNA.
      2. Works only on E. coli UDG (NEB #M0280).  
      3. Use equal or more units of UGI than units of UDG in the original reaction.
      4. Active in NEBuffers 1-4.  (NEB #B7001, NEB #B7002, NEB #B7003, NEB #B7004)
      5. UGI is extremely thermostable, retaining > 95% activity after boiling for 10 minutes.

    • References

      1. Lindahl, T. et al. (1977). J. Biol.Chem.. 252, 3286-3294.
      2. Wang, Z., et al. (1991). Gene. 99, 31-37.
      3. Bennett, S. E. and Mosbaugh, D. W. (1992). J. Biol. Chem.. 267, 22512-22521.

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