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  • Q5® Reaction Buffer Pack


    New England Biolabs supplies a 5X reaction buffer and supplemental reagent for use with Q5® High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Q5® High GC Enhancer5X

    Properties and Usage

    Storage Temperature


    Quality Control

    Quality Assurance Statement

    • This reaction buffer and supplement are free of detectable nucleases.


    1. Reaction Conditions:
      At a 1X concentration the reaction buffer contains 2 mM MgCl2 and assures optimal activity for Q5 High-Fidelity DNA Polymerase and Q5 Hot Start High-Fidelity DNA Polymerase.


    1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404. PubMedID: PMC3567689
    2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE 
      OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247
    3. Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
    2. When should I add the High GC Enhancer?
    3. There is a precipitate in the bottom of the buffer tube. Is this normal?
    4. Do other polymerases work in Q5® Reaction Buffer?