rCutSmart™ Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
B6004S     -20       rCutSmart™ Buffer B6004SVIAL -20 4 x 1.25 ml 10 X

Companion Products

• Recombinant Albumin, Molecular Biology Grade (Animal-free)
• Nuclease-free Water
• NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer
• Double Digests
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• NEBuffer Comparison Chart (1X)

• Double Digest Finder
• Enzyme Finder
• NEBcloner®
• NEBioCalculator®

1. What is the difference between CutSmart Buffer  and rCutSmart Buffer?
2. Why is my Restriction Enzyme not cutting DNA?
3. Why do I see additional DNA bands on my gel after a restriction digest?
4. Why do I see a DNA smear on an agarose gel after a restriction digest?
5. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
6. The buffer arrived thawed.  Are the buffer and the enzyme still active?
7. How should the NEBuffer be used?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• B6004S_V_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• B6004S_v1_10123660
• B6004S_v1_10104774
• B6004S_v1_10138489
• B6004S_v1_10141083
• B6004S_v1_10148896
• B6004S_v1_10156547
• B6004S_v1_10160661
• B6004S_v1_10164828
• B6004S_v1_10173159
• B6004S_v1_10175649
• B6004S_v1_10180571

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• rCutSmart™ Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.