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University of Texas, Austin

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NEB # Buffers Size Stockroom #
B9000S BSA, Molecular Biology Grade 12 mg B9000S
B7025S Gel Loading Dye, Purple (6X), no SDS 4 ml B7025S
NEB # DNA Modifying Enzymes & Cloning Technologies Size Stockroom #
E2611S Gibson Assembly® Master Mix 10 reactions E2611S
E2621S NEBuilder® HiFi DNA Assembly Master Mix 10 reactions E2621S
M0530L Phusion® High-Fidelity DNA Polymerase 500 units M0530L
M0530S Phusion® High-Fidelity DNA Polymerase 100 units M0530S
E0552S Q5® Site-Directed Mutagenesis Kit (Without Competent Cells) 10 reactions E0552S
M2200S Quick Ligation™ Kit 30 reactions M2200S
M0202T T4 DNA Ligase 20,000 units M0202T
M0202S T4 DNA Ligase 20,000 units M0202S
M0202M T4 DNA Ligase 100,000 units M0202M
M0202L T4 DNA Ligase 100,000 units M0202L
M0318S T7 DNA Ligase 100,000 units M0318S
M0208L Taq DNA Ligase 10,000 units M0208L
M5505L USER® Enzyme 250 units M5505L
NEB # Epigenetics Size Stockroom #
R0176S DpnI 1,000 units R0176S
R0176L DpnI 5,000 units R0176L
R3138S SalI-HF® 2,000 units R3138S
NEB # Library Preparation for Next Generation Sequencing Size Stockroom #
E7490S NEBNext® Poly(A) mRNA Magnetic Isolation Module 24 reactions E7490S
NEB # Markers & Ladders Size Stockroom #
N3232L 1 kb DNA Ladder 1 ml N3232L
N3232S 1 kb DNA Ladder 0.2 ml N3232S
N3231L 100 bp DNA Ladder 0.5 ml N3231L
N3231S 100 bp DNA Ladder 0.1 ml N3231S
N3236S 50 bp DNA Ladder 0.1 ml N3236S
N3233S Low Molecular Weight DNA Ladder 0.1 ml N3233S
N0552S Quick-Load® Purple 1 kb DNA Ladder 1.25 ml N0552S
N0550S Quick-Load® Purple 1 kb Plus DNA Ladder 1.25 ml N0550S
N0551S Quick-Load® Purple 100 bp DNA Ladder 1.25 ml N0551S
NEB # Nucleic Acid Purification Size Stockroom #
T1020S Monarch® DNA Gel Extraction Kit 50 preps T1020S
T1020L Monarch® DNA Gel Extraction Kit 250 preps T1020L
T1030L Monarch® PCR & DNA Cleanup Kit (5 μg) 250 preps T1030L
T1010S Monarch® Plasmid Miniprep Kit  50 preps T1010S
T1010L Monarch® Plasmid Miniprep Kit  250 preps T1010L
NEB # PCR, Polymerases & Amplification Technologies Size Stockroom #
M0210S DNA Polymerase I, Large (Klenow) Fragment 200 units M0210S
E3005S Luna® Universal One-Step RT-qPCR Kit 200 reactions E3005S
E3006S Luna® Universal Probe One-Step RT-qPCR Kit 200 reactions E3006S
M3004S Luna® Universal Probe qPCR Master Mix 200 rxn (2 x 1 ml) M3004S
M3003S Luna® Universal qPCR Master Mix 200 rxn (2 x 1 ml) M3003S
M0541L NEBNext® High-Fidelity 2X PCR Master Mix 250 reactions M0541L
M0480S OneTaq® DNA Polymerase 200 units M0480S
M0480L OneTaq® DNA Polymerase 1,000 units M0480L
M0491L Q5® High-Fidelity DNA Polymerase 500 units M0491L
M0491S Q5® High-Fidelity DNA Polymerase 100 units M0491S
M0494S Q5® Hot Start High-Fidelity 2X Master Mix 100 reactions (50 μl vol) M0494S
M0493L Q5® Hot Start High-Fidelity DNA Polymerase 500 units M0493L
M0203S T4 DNA Polymerase 150 units M0203S
M0267S Taq DNA Polymerase with ThermoPol® Buffer 400 units M0267S
M0267L Taq DNA Polymerase with ThermoPol® Buffer 2,000 units M0267L
NEB # Protein Tools Size Stockroom #
P7706S Blue Prestained Protein Standard, Broad Range (11-190 kDa) 150 gel lanes P7706S
P7712S Color Prestained Protein Standard, Broad Range (11–245 kDa) 150 gel lanes P7712S
P8107S Proteinase K, Molecular Biology Grade 2 x 1 ml vials P8107S
NEB # Restriction Endonucleases Size Stockroom #
R0117S AatII 500 units R0117S
R0558S AscI 500 units R0558S
R3136S BamHI-HF® 10,000 units R3136S
R3539S BbsI-HF® 300 units R3539S
R3535S BsaI-HF® 1,000 units R3535S
R0580S BsmBI 200 units R0580S
R0575S BsrGI 1,000 units R0575S
R0197S ClaI 1,000 units R0197S
R3510S DraIII-HF® 1,000 units R3510S
R3101S EcoRI-HF® 10,000 units R3101S
R3195S EcoRV-HF® 4,000 units R3195S
R3104S HindIII-HF® 10,000 units R3104S
R0105S HpaI 500 units R0105S
R3142S KpnI-HF® 4,000 units R3142S
R3589S MfeI-HF® 500 units R3589S
R3193S NcoI-HF® 1,000 units R3193S
R0111S NdeI 4,000 units R0111S
R3131S NheI-HF® 1,000 units R3131S
R3189L NotI-HF® 2,500 units R3189L
R3189S NotI-HF® 500 units R3189S
R0547S PacI 250 units R0547S
R0547L PacI 1,250 units R0547L
R0560S PmeI 500 units R0560S
R3140S PstI-HF® 10,000 units R3140S
R3156S SacI-HF® 2,000 units R3156S
R0123S SfiI 3,000 units R0123S
R0133S SpeI 500 units R0133S
R3133S SpeI-HF® 500 units R3133S
R3182S SphI-HF® 500 units R3182S
R0145S XbaI 3,000 units R0145S
R0146S XhoI 5,000 units R0146S
R0180S XmaI 500 units R0180S
NEB # RNA Reagents Size Stockroom #
M0290S Alkaline Phosphatase, Calf Intestinal (CIP) 1,000 units M0290S
M0289S Antarctic Phosphatase 1,000 units M0289S
N0440S dATP Solution 25 μmol N0440S
N0447S Deoxynucleotide (dNTP) Solution Mix 8 μmol of each N0447S
N0446S Deoxynucleotide (dNTP) Solution Set 25 μmol of each N0446S
M0303S DNase I (RNase-free) 1,000 units M0303S
T2010S Monarch® Total RNA Miniprep Kit 50 preps T2010S
E6560S ProtoScript® II First Strand cDNA Synthesis Kit 30 reactions E6560S
M0368S ProtoScript® II Reverse Transcriptase 4,000 units M0368S
M0297S RNase H 250 units M0297S
M0314S RNase Inhibitor, Murine 3,000 units M0314S
M0371S Shrimp Alkaline Phosphatase (rSAP) 500 units M0371S
M0201S T4 Polynucleotide Kinase 500 units M0201S
NEB # Sample Preparation for Next Generation Sequencing Size Stockroom #
E7760L NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® 96 reactions E7760L

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