Phusion® High-Fidelity PCR Master Mix with HF Buffer
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.
- 50X higher fidelity compared to Taq
- Robust reactions - maximal success with minimal optimization
- Master mix format with HF buffer ideal for a wide range of AT/GC content
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Product Information
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.
Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995. 
A 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacturer's recommended conditions. Extension times are indicated (in minutes). Ladder L is a 1 kb DNA Ladder. Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.- This product is related to the following categories:
- Phusion® High-Fidelity DNA Polymerases Products,
- Master Mixes Products
- This product can be used in the following applications:
- Long Range PCR,
- Fast PCR,
- High-Fidelity PCR,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR, PCR
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