T4 RNA Ligase 2, truncated KQ

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0373S     -20       T4 RNA Ligase 2, truncated KQ M0373SVIAL -20 1 x 0.01 ml 200,000 units/ml   T4 RNA Ligase Reaction Buffer B0216SVIAL -20 1 x 1.5 ml 10 X   50% PEG 8000 B1004SVIAL -20 1 x 1 ml 1 X
M0373L     -20       T4 RNA Ligase 2, truncated KQ M0373LVIAL -20 1 x 0.05 ml 200,000 units/ml   T4 RNA Ligase Reaction Buffer B0216SVIAL -20 1 x 1.5 ml 10 X   50% PEG 8000 B1004SVIAL -20 1 x 1 ml 1 X

Application Features

• Ligate a pre-adenylated DNA or RNA sequence tag to any RNA 3´-end 
• Join a single stranded adenylated primer to small RNAs for cDNA library creation 
• Join a single stranded adenylated primer to RNA for strand-specific cDNA library construction.

Companion Products

• RNase Inhibitor, Murine

1. In our tests, this ligase can be used interchangeablywith T4 Rnl2tr (NEB #M0242). Ligationactivity is stimulated by adding PEG (See FAQ).

1. Yin, S., Ho, C.K. and Shuman S. (2003). J. Biol. Chem.. 278, 17601-17608.
2. Hafner, M., et al. (2008). Methods. 44, 3-12.
3. Viollet, S., et al. (2011). BMC Biotechnol. 11, 72.
4. Zhuang, F., et al. (2012). Nucleic Acids Res.. 40(7), e54.

1. Protocol for ligation of the 3´ OH of RNA to the 5´ pre-adenylated DNA with T4 RNA Ligase 2 truncated KQ (NEB #M0373)

• Properties of DNA and RNA Ligases
• RNA Ligase Selection Chart
• Substrate-based Ligase Selection Chart

1. Is T4 RNA Ligase 2, truncated KQ different than T4 RNA Ligase 2, truncated K227Q?
2. Are there differences in ligation efficiency for T4 RNA Ligase 2, truncated KQ, T4 RNA Ligase 2, truncated 227Q, and T4 RNA Ligase 2, truncated KQ?
3. What can T4 RNA Ligase 2, truncated KQ ligate?
4. Does PEG stimulate ligation efficiency?
5. How can I increase ligation efficiency?
6. Can T4 RNA Ligase 2, truncated KQ be used in other NEBuffers?
7. The molecular weight of T4 RNA Ligase 2, truncated KQ is 71.6 kDa. Is it a fusion?
8. Is PEG 8000 available for purchase?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0373S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0373S_L_v1_0031804
• M0373S_L_v1_0031710
• M0373L_v1_10008697
• M0373S_v1_10008698
• M0373L_v2_10028372
• M0373S_v2_10028369
• M0373L_v2_10041612
• M0373S_v2_10041614
• M0373L_v2_10052059
• M0373L_v2_10057255
• M0373S_v2_10052062
• M0373L_v2_10062650
• M0373S_v2_10065560
• M0373L_v2_10070020
• M0373L_v2_10084293
• M0373S_v2_10084296
• M0373L_v2_10105435
• M0373S_v2_10112133
• M0373L_v2_10122069
• M0373L_v2_10127451
• M0373S_v2_10127450
• M0373L_v2_10140375
• M0373S_v2_10146863
• M0373L_v2_10166706
• M0373S_v2_10166708
• M0373L_v2_10183116
• M0373S_v2_10196881
• M0373L_v2_10208820
• M0373S_v2_10219415
• M0373L_v2_10242298
• M0373S_v2_10242317
• M0373L_v2_10269184

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T4 RNA Ligase 2, truncated KQ
• T4 RNA Ligase Reaction Buffer
• PEG 8000

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.