NEBNext^® Multiplex Oligos for Illumina^® (Index Primers Set 3)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7710S     -20       NEBNext Index 26 Primer for Illumina E7713AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 28 Primer for Illumina E7714AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 29 Primer for Illumina E7715AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 31 Primer for Illumina E7717AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 32 Primer for Illumina E7718AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 33 Primer for Illumina E7719AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 34 Primer for Illumina E7720AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 35 Primer for Illumina E7721AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 36 Primer for Illumina E7722AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Universal PCR Primer for Illumina E6861AVIAL -20 1 x 0.12 ml 10 µM   NEBNext Adaptor for Illumina E7337AVIAL -20 1 x 0.24 ml Not Applicable   USER® Enzyme E7338AVIAL -20 1 x 0.072 ml Not Applicable   NEBNext Index 42 Primer for Illumina E7736AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 24 Primer for Illumina E7592AVIAL -20 1 x 0.01 ml 10 µM   NEBNext Index 17 Primer for Illumina E7585AVIAL -20 1 x 0.01 ml 10 µM
E7710L     -20       NEBNext Adaptor for Illumina E7337AAVIAL -20 1 x 0.96 ml Not Applicable   USER® Enzyme E7338AAVIAL -20 1 x 0.288 ml Not Applicable   NEBNext Universal PCR Primer for Illumina E6861AAVIAL -20 1 x 0.48 ml 10 µM   NEBNext Index 17 Primer for Illumina E7585AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 24 Primer for Illumina E7592AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 26 Primer for Illumina E7713AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 28 Primer for Illumina E7714AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 29 Primer for Illumina E7715AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 31 Primer for Illumina E7717AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 32 Primer for Illumina E7718AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 33 Primer for Illumina E7719AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 34 Primer for Illumina E7720AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 35 Primer for Illumina E7721AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 36 Primer for Illumina E7722AAVIAL -20 1 x 0.04 ml 10 µM   NEBNext Index 42 Primer for Illumina E7736AAVIAL -20 1 x 0.04 ml 10 µM

Companion Products

• NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®
• NEBNext® Ultra™ II Ligation Module 
• NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
• NEBNext® Ultra Ligation Module
• NEBNext® Quick Ligation Module
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II DNA Library Prep with Sample Purification Beads
• NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina
• NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads

• E7710_SingleIndex_IlluminaLRM
• Sample Sheet Index Sequences E7710

• NEBNext® Multiplex Oligos Selection Chart

1. Do you have a Sample Sheet with the index sequences for NEBNextMultiplex Oligos for Illumina (Set 3, #E7710) that can be used in theIllumina Experiment Manager (IEM)?
2. Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
3. Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
4. Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
5. How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
6. Are these reagents available in a customized format, larger format or 96 well formats?
7. Can USER Enzyme be purchased separately?
8. Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
9. How do I use the NEB provided Sample Sheet for my specific instrument?
10. Are the NEBNext Oligos for Illumina compatible with single read and paired end sequencing?
11. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7710S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7710S_v1_10010787
• E7710L_v1_10013218
• E7710L_v1_10013268
• E7710S_v1_10015724
• E7710S_v1_10017803
• E7710S_v1_10021150
• E7710L_v1_10025711
• E7710S_v1_10027450
• E7710L_v1_10032129
• E7710S_v1_10032688
• E7710L_v1_10022039
• E7710S_v1_10022023
• E7710S_v1_10042709
• E7710L_v1_10045158
• E7710S_v1_10046532
• E7710S_v1_10050090
• E7710L_v1_10048920
• E7710S_v1_10050506
• E7710L_v1_10057649
• E7710S_v1_10037769
• E7710L_v1_10060390
• E7710S_v1_10063372
• E7710L_v1_10065035
• E7710S_v1_10066473
• E7710L_v1_10071226
• E7710S_v1_10071799
• E7710L_v1_10072617
• E7710S_v1_10077073
• E7710L_v1_10059367
• E7710S_v1_10081224
• E7710L_v1_10084764
• E7710S_v1_10086198
• E7710L_v1_10088760
• E7710S_v1_10092040
• E7710L_v1_10094263
• E7710S_v1_10094729
• E7710L_v1_10097188
• E7710L_v1_10070855
• E7710S_v1_10100998
• E7710S_v1_10070840
• E7710S_v1_10110631
• E7710L_v1_10112757
• E7710S_v1_10115815
• E7710S_v1_10121661
• E7710L_v1_10127713
• E7710S_v1_10130501
• E7710S_v1_10135714
• E7710L_v1_10095997
• E7710S_v1_10095984
• E7710S_v1_10146405
• E7710L_v1_10148254
• E7710L_v1_10155927
• E7710S_v1_10155607
• E7710S_v1_10162390
• E7710L_v1_10166214
• E7710S_v1_10169492
• E7710L_v1_10170983
• E7710L_v1_10175299
• E7710S_v1_10175178
• E7710L_v1_10178530
• E7710S_v1_10136550
• E7710S_v1_10184181
• E7710L_v1_10189576
• E7710L_v1_10196499
• E7710S_v1_10198070
• E7710L_v1_10172564
• E7710S_v1_10172583
• E7710L_v1_10208562
• E7710S_v1_10213921
• E7710L_v1_10193809
• E7710S_v1_10225811
• E7710S_v1_10193824
• E7710L_v1_10233054
• E7710S_v1_10243948
• E7710S_v1_10233068

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext Index 26 Primer for Illumina
• NEBNext Index 28 Primer for Illumina
• NEBNext Index 29 Primer for Illumina
• NEBNext Index 31 Primer for Illumina
• NEBNext Index 32 Primer for Illumina
• NEBNext Index 33 Primer for Illumina
• NEBNext Index 34 Primer for Illumina
• NEBNext Index 35 Primer for Illumina
• NEBNext Index 36 Primer for Illumina
• NEBNext Universal PCR Primer for Illumina
• NEBNext Adaptor for Illumina
• USER^® Enzyme
• NEBNext Index 42 Primer for Illumina
• NEBNext Index 24 Primer for Illumina
• NEBNext Index 17 Primer for Illumina

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is covered by one or more patents.

This product is licensed from Bio-Rad Laboratories, Inc. under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, 7,919,296, 8,232,078, 8,367,376, 8,415,129, 8,445,249, 8,470,573, 8,476,045, 8,895,283 and 8,900,846 and corresponding patents in other countries for use only in: (a) standard (non-real time) PCR in the research field only, but not  digital PCR; (b) real-time PCR for internal product research and development purposes only, and not for sales to end-users within the research field; (c) any in vitro diagnostics application, including applications using real-time PCR but not digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification and the production of synthetic DNA.