PNGase F – Designed for your application
The industry standard for purity

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All of NEB's enzymes undergo extensive quality controls to ensure purity and lot-to-lot consistency. NEB glycosidases, including PNGase F products, are no different, and are tested for contaminating exoglycosidase, endoglycosidase and proteolytic activities. Unlike other suppliers, NEB uses fluorescently-labeled oligosaccharides to screen for contaminating activities, which increases sensitivity. Consistency and reproducibility are tested across three lots, ensuring optimized activity for your application.
 
 

Highlights

NEB is certified to both ISO 9001:2008 and ISO 13485:2003 standards for quality management systems.

• Recombinant enzymes; manufactured in-house
• All glycosidase products from NEB exhibit ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
• Stringent quality control testing includes assays for detecting contaminating exoglycosidase, endoglycosidase and proteolytic activity

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Quality data

Every NEB enzyme is subjected to a quality control assay to determine protein purity. The physical purity is assessed by both SDS-PAGE and intact ESI-MS analysis. An example of this is shown below, using PNGase F. It is imperative to hold these enzymes to a high quality standard, as they must be compatible with the needs of regulated applications and standardized procedures, as well being free of contaminants and side activities. High-quality products are also necessary to meet challenges associated with glycoproteomic analyses.

• Download our latest white paper “Redesigning glycosidase manufacturing quality for pharmaceutical and clinical applications”
• Learn more about NEB's quality philosophy.
• View our press release about discussing our plans for a GMP facility.

Purity determination by SDS-PAGE and ESI-MS analysis

 
 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain application.