FspEI
neb31 cloned at NEB recombinant dil_B 37 80 Heat Epi

FspEI was discontinued on September 15, 2023. MspJI (NEB #R0661) may be a suitable replacement.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.


  • Catalog # R0662 was discontinued on September 15, 2023
NEB restriction endonuclease that recognizes the sequence CCNNNNNNNNNNNN^NNNN_
  • 100% activity in rCutSmart Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Specificity to epigenetically-relevant DNA modifications (5-mC and 5-hmC)

  • Restriction Enzyme Cut Site: CC(12/16)

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