HomeFAQsHow can I minimize off target or star activity?
FAQ: How can I minimize off target or star activity?
Star Activity, including digestion of non-methylated DNA substrates, may result from non-optimal reaction conditions such as extended digestion time, high enzyme or activator concentration or a glycerol concentration of > 5%. Optimization of the amount of enzyme needed to achieve efficient specific cutting while avoiding star activity may be required for each substrate DNA.
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