Endonuclease IV

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0304S     -20       Endonuclease IV M0304SVIAL -20 1 x 0.1 ml 10,000 units/ml   NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X
M0304L     -20       Endonuclease IV M0304LVIAL -20 1 x 0.5 ml 10,000 units/ml   NEBuffer™ 3 B7003SVIAL -20 1 x 1.25 ml 10 X

Application Features

• Single cell gel electrophoresis (Comet assay)(2,3,4)
• Alkaline elution (5)
• Alkaline unwinding (6)

Materials Sold Separately

• NEBuffer 3

1. Recommended Dilution for the Comet Assay: 1.10⁴ to 1:10⁵(2,3,4,7). For a protocol please visit: http://cometassay.com
2. The Endo IV optimum buffer is 1X NEBuffer 3 (NEB #B7003). The enzyme has 100% activity in NEBuffer 2 (NEB #B7002) and 25% activity in CutSmart Buffer (NEB #B7204). The use of Endo IV in NEBuffer 1 (NEB #B7001) is not recommended. 
3. The enzyme is also active in ThermoPol^® Reaction Buffer (NEB #B9004).

1. Levin, J. et al. (1988). J. Gen. Physiol. . 33, 349-362.
2. Singh, N. et al. (1961). Experimental Cell  Research . 175, 184-191.
3. Collins, A. etal. (1993). Carcinogenesis . 14, 1733-1735.
4. Collins, A. et al. (1996). Environmental Health Perspectives . 104, 465-469.
5. Pflaum, M. et al. (1998). Free Rad. Res. 29, 585-594.
6. Hartwig, A. et al. (1996). Toxicology Letters. 88, 85-90.
7. Marks, K., New England Biolabs, Inc. unpublished observations.

1. Protocol for Endonuclease IV (M0304)

• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of Endonuclease IV in the NEBuffers?
2. What is the molecular weight for Endonuclease IV?
3. Does Endonuclease IV cleave ssDNA?
4. Will the 5' terminus left by an Endonuclease IV cleavage be suitable for subsequent ligation by T4 DNA ligase?
5. In addition to AP sites, does Endonuclease IV have cleavage activity on other types of DNA damage?
6. Can an abasic site be created and then cleaved with Endonuclease IV in the same reaction? How active is Uracil-DNA-Glycosylase (cat# M0280S) in Endonuclease IV buffer?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0304S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0304L_v1_10028878
• M0304S_v1_10028880
• M0304L_v1_10033531
• M0304L_v1_10038477
• M0304S_v1_10033533
• M0304L_v1_10043663
• M0304S_v1_10043661
• M0304S_v1_10049415
• M0304L_v1_10055589
• M0304L_v1_10057940
• M0304S_v1_10057939
• M0304L_v1_10062103
• M0304S_v1_10068189
• M0304L_v1_10075674
• M0304S_v1_10075675
• M0304S_v1_10081654
• M0304L_v1_10081657
• M0304L_v1_10088354
• M0304S_v1_10088353
• M0304L_v1_10094368
• M0304L_v1_10096983
• M0304S_v1_10094370
• M0304S_v1_10104804
• M0304S_v1_10107481
• M0304L_v1_10107479
• M0304S_v1_10117728
• M0304S_v1_10139695
• M0304L_v1_10139692
• M0304L_v1_10159644
• M0304S_v1_10159645
• M0304L_v1_10170596
• M0304S_v1_10176767
• M0304L_v1_10176768
• M0304S_v1_10195664
• M0304S_v1_10206698
• M0304L_v1_10195665
• M0304S_L_v1_0041803
• M0304L_v1_10220023
• M0304L_v1_10226645
• M0304S_v1_10221028
• M0304L_v1_10235099
• M0304S_v1_10245584
• M0304L_v1_10249376
• M0304S_v1_10262135
• M0304L_v1_10269456
• M0304L_v1_10279088
• M0304S_v1_10279086
• M0304L_v1_10289219
• M0304L_v1_10297870
• M0304S_v1_10298684
• M0304S_v1_10309443
• M0304L_v1_10322996
• M0304S_v1_10322995
• M0304L_v1_10337090
• M0304S_v1_10330912
• M0304L_v1_10340584

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Endonuclease IV
• NEBuffer™ 3

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.