Q5® High-Fidelity DNA Polymerases
Find the right Q5 product for your polymerase chain reaction (PCR)
What are the differences between the numerous Q5® Polymerase products available?
The five features of Q5 High-Fidelity DNA Polymerase
- Extremely low error rates
At ~280X higher than Taq, Q5 offers unparalleled fidelity for your most important samples. For information on how fidelity affects PCR‑induced errors and experimental outcomes, explore the PCR Fidelity Estimator tool.
- Robust amplification with minimal optimization
High specificity and yield are absolute requirements for today's molecular biology techniques.
- Superior coverage for a broad range of amplicons, regardless of GC content
While other DNA polymerases can have difficulty amplifying high-GC or high-AT amplicons, Q5 displays superior performance for a wide range of templates.
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Shorter PCR protocols
Achieve precision without sacrificing speed. Q5's unique design incorporating the SSo7d processivity-enhancing domain enables shorter extension times, as low as 5 seconds per kb. Additionally, the aptamer-based hot start requires no initial denaturation step and enables room temperature setup.
For added convenience, Q5 products are compatible with single (i.e., "universal") annealing temperature protocols. Please see our application note for more information: Universal Annealing Temperature in PCR and its Impact on Amplification Results. - Amplify templates up to 30 kb
When speed is a priority or DNA targets are long or complex, Q5‑XT provides a faster, more robust amplification solution while maintaining the performance you expect of Q5. Simple templates up to 30 kb and complex templates up to 20 kb can be amplified with high accuracy.
Phusion™ DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.
THERMO SCIENTIFIC™ and PHUSION™ are registered trademarks and property of Thermo Fisher Scientific used under license.
ACCUPRIME™ and PLATINUM™ are trademarks of Thermo Fisher Scientific
PFUULTRA® and AGILENT are registered trademarks of Agilent Technologies, Inc.
MILLIPORE SIGMA® is a registered trademark of Merck KGAA
LABCHIP® is a registered trademark of Caliper Life Sciences, part of Perkin Elmer, Inc.
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.
Choose Product:
Choose Type:
- PCR Using NEBNext® High-Fidelity 2X PCR Master Mix (NEB #M0541)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (NEB #M0493)
- PCR Using Q5® Hot Start High-Fidelity 2X Master Mix (NEB #M0494)
- PCR Using Q5® High-Fidelity 2X Master Mix (NEB #M0492)
- PCR Optimization (E0555)
- Protocol for a Routine PCR (E0555)
- Protocol for a PCR reaction using NEBNext® Q5® Hot Start HiFi PCR Master Mix (M0543)
- Protocol for a PCR reaction using NEBNext® Ultra™ II Q5® Master Mix (M0544)
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Using aptamers to control enzyme activity: Hot Start Taq and beyond
This article describes the use of engineered oligonucleotides known as aptamers in regulating the enzyme activity of polymerases and reverse transcriptases.
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Polymerase Fidelity: What is it, and what does it mean for your PCR?
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Understanding Variability in DNA Amplification Reactions
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Anatomy of a Polymerase - How Function and Structure are Related
Read about the relationship between Polymerase structure and function when copying DNA.
- PCR Reagents Brochure
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
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