RNA Analysis

RNA Analysis

RNA is a polynucleotide that is typically transcribed from a DNA template. Historically, its only known function was involvement in the translation of genetic information into proteins, either through encoding the sequence of the protein (messenger RNA; mRNA), bringing amino acids to the ribosome (transfer RNA; tRNA), or by being a component of the ribosome translational machinery (ribosomal RNA; rRNA). Recently, technological advances and innovative research in the field of RNA analysis, have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions. These findings have helped usher in a renaissance of RNA-focused research in biology.

  1. In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

RNA Analysis includes these areas of focus:

RNA Characterization & Modification
RNA Structural Analysis
RNA Probe Generation
cDNA Synthesis & RT-PCR
RNA Cloning
RNA Expression Analysis
RNA Processing & Analysis
RNA Library Prep for NGS
RNA Detection & Isolation
In vitro Synthesis & Processing

Protocols for RNA Analysis

    Publications related to RNA Analysis:

  1. Fuchs, R.T., Sun, Z., Zhuang, F., et al. (2015). Bias in ligation-based small RNA sequencing library construction is determined by adaptor and RNA structure PLoS One. 10(5), PubMedID: 25942392, DOI: 10.1371/journal.pone.0126049

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This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

RNA Reagents Overview

Reagents for RNA Sample Preparation

NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.

RNA Polymerase Selection Chart

NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

RNA Ligase Selection Chart

NEB offers a variety of ligases for RNA research with unique specificities.

cDNA Synthesis Selection Chart

NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.