RNA Analysis
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  • RNA Analysis

    RNA is a polynucleotide that is typically transcribed from a DNA template. Historically, its only known function was involvement in the translation of genetic information into proteins, either through encoding the sequence of the protein (messenger RNA; mRNA), bringing amino acids to the ribosome (transfer RNA; tRNA), or by being a component of the ribosome translational machinery (ribosomal RNA; rRNA). Recently, technological advances and innovative research in the field of RNA analysis, have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions. These findings have helped usher in a renaissance of RNA-focused research in biology.

    • In Vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

      This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

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    RNA Analysis includes these areas of focus:

    RNA Cloning
    RNA Characterization & Modification
    RNA Structural Analysis
    RNA Probe Generation
    Transfection
    In vitro Synthesis & Processing
    cDNA Synthesis & RT-PCR
    RNA Expression Analysis
    RNA Processing & Analysis
    RNA Library Prep for NGS
    RNA Detection & Isolation

    RNA Reagents Overview

    Reagents for RNA Sample Preparation

    NEB offers a wide variety of reagents to facilitate front-end processing of RNA for downstream applications including next-generation sequencing, library construction and microarrays.

    RNA Polymerase Selection Chart

    NEB offers RNA polymerases that can be used for in vitro synthesis of RNA for a wide variety of downstream applications. Additional polymerases are offered for the generation of untemplated homoribopolymeric tails for reverse transcription or labeling.

    RNA Ligase Selection Chart

    NEB offers a variety of ligases for RNA research with unique specificities.

    cDNA Synthesis Selection Chart

    NEB offers several reagents for cDNA synthesis for use in applications including qPCR and qRT-PCR. For your convenience, reagents are available as kits or standalone products, depending on your needs.