RNA Synthesis In vitro Transcription (IVT)

Tools to Take You from Template to Transcript

NEB is at the cutting edge of discovery, engineering, and application of enzymes for RNA synthesis. Our tools take you from template to transcript, supported by the scientists who develop them. Our enzymology expertise and reagent manufacturing support maximize the potential of rapid mRNA technology at every scale.   

In vitro synthesis of single-stranded RNA molecules is a widely used laboratory procedure that is critical to RNA research, as well as to RNA biopharmaceuticals. This technique is versatile in that it allows the researcher to tailor synthesis and introduce modifications to produce a transcript. Downstream applications include biochemical and molecular characterization of RNA for RNA-protein interactions and structural analyses, generation of RNA aptamers, synthesis of functional mRNAs for expression, and generation of small RNAs for alteration of gene expression (e.g., guide RNAs, RNAi). Furthermore, the use of in vitro synthesized RNA has been instrumental in the development of RNA vaccines and CRISPR/Cas9 genome editing tools, generation of pluripotent stem cells, the screening of RNA inhibitors, as well as the development of RNA amplification-based diagnostics.

High-yield robust reactions require optimization of each reaction component. NEB offers six in vitro RNA synthesis kits, all of which have been optimized to generate reproducible yields of quality RNA. Additionally, individual components can be purchased for in vitro transcription (IVT) and mRNA capping.

NEB’s portfolio of research-grade and GMP-grade* reagents enables bench-scale to commercial-scale mRNA manufacturing. Our optimized HiScribe^® kits enable convenient in vitro transcription workflows. When it is time to scale up and optimize reaction components, our standalone reagents are readily available in formats matching our GMP-grade offering, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.

Template Generation		In vitro Transcription		RNA Capping		Poly(A) Tailing		RNAPurification
Q5® High-Fidelity DNA Polymerase		HiScribe T7 mRNA Kit with CleanCap® Reagent AG				E. coli Poly(A) Polymerase		Monarch® RNA Cleanup Kit (10 μg)
		HiScribe™ T7 ARCA mRNA Synthesis Kit (with tailing)
phi29 DNA Polymerase		HiScribe T7 ARCA mRNA Synthesis Kit						Monarch RNA Cleanup Kit (50 μg)
TelN Protelomerase		HiScribe T7 High Yield RNA Synthesis Components		Faustovirus Capping Enzyme				Monarch RNA Cleanup Kit (500 μg)
				Vaccinia Capping System
dNTP solution mixes		HiScribe T7 Quick High Yield RNA Synthesis Kit		mRNA Cap 2′-O-Methyltranferase				Lithium Chloride
BspQI*		HiScribe SP6 High Yield RNA Synthesis Kit		ARCA and other mRNA cap analogs
DNA Assembly: NEBuilder HiFi DNA Assembly Golden Gate Assembly		T3 & SP6 RNA Polymerases
		T7 RNA Polymerase
		Hi-T7 RNA Polymerase
		Companion Products						Companion Products
		RNase Inhibitor (Murine)						Monarch RNA Cleanup Binding Buffer
		RNase Inhibitor (Human Placental)						Monarch RNA Cleanup Wash Buffer
		Pyrophosphatase, Inorganic (E. coli)						Nuclease-free Water
		Pyrophosphatase, Inorganic (Yeast)
		DNase I (RNase-free)
		DNase I-XT
		NTPs

= available in GMP-grade

= available in GMP-grade

* "GMP-grade" is a branding term NEB uses to describe reagents manufactured at NEB’s Rowley facility. The Rowley facility was designed to manufacture reagents under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Reagents manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.