NEBNext® Ultra™ II RNA Library Prep

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.

Products include:

Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the "dUTP method") and non-directional library prep, with the option of SPRISelect® beads for size-selection and clean-up steps.

Why consider the NEBNext Ultra II Directional RNA Library Prep Kit?

  • Get more of what you need, with the highest library yields
  • Generate high-quality libraries even with limited amounts of RNA:
    • 10 ng – 1 µg Total RNA  (poly(A) mRNA workflow
    • 10 ng – 1 µg Total RNA (rRNA depletion workflow)
  • Minimize bias, with fewer PCR cycles required
  • Increase the complexity and transcript coverage of your libraries
  • Maximize flexibility by ordering reagents for your specific workflow needs, including directional and non-directional kits, rRNA depletion and poly(A) mRNA isolation reagents, and adaptors and primers (12- and 96-index) sold separately
  • Enjoy the reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need
  • Save time with streamlined workflows, reduced hands-on time, and automation compatibility
  • Rely on robust performance, even with low quality RNA, including FFPE


Are you looking for non-directional library prep?

View performance data for our NEBNext Ultra II RNA Library Prep Kit: 


NEBNext Ultra II Directional RNA produces the highest yields, from a range of input amounts
ULtra II yields
Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human Reference RNA (Agilent #740000) and libraries were made using the NEBNext Ultra II Directional RNA kit, Kapa Stranded mRNA-Seq kit, Kapa mRNA HyperPrep kit and Illumina TruSeq Stranded mRNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. Error bars indicate standard deviation. Library yields were assessed using the Agilent® Bioanalyzer®.

This content covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

KAPA® is a registered trademark of Kapa Biosystems
ILLUMINA® and TRUSEQ® are registered trademarks of Illumina, Inc.
AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.

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Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.