CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and CRISPR-associated (Cas) genes are essential in adaptive immunity in select bacteria and archaea, enabling the organisms to respond to and eliminate invading genetic material.
FAQs for Genome Editing
Protocols for Genome Editing
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporation
- EnGen® sgRNA Synthesis Kit, S. pyogenes Protocol (NEB #E3322)
- NEBuilder HiFi DNA Assembly Reaction Protocol
- NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol
- NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol (E2621)
- NEBuilder® HiFi DNA Assembly Electrocompetent Transformation Protocol
- NEBuilder® HiFi Electrocompetent Transformation Protocol (E2621)
- PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)
- sgRNA Synthesis Using the HiScribe™ Quick T7 High Yield RNA Synthesis Kit (NEB #E2050)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (NEB #M0386)
Genome Editing Brochure
Learn more about NEB's complete solution for your genome editing needs, starting with our new Cas9 Nuclease (S. pyogenes) for CRISPR studies.
- CRISPR/Cas9 and Targeted Genome Editing: A New Era in Molecular Biology
Other Tools & Resources
- Zheng Hu, Wencheng Ding, Da Zhu, Lan Yu, Xiaohui Jiang, Xiaoli Wang, Changlin Zhang, Liming Wang, Teng Ji, Dan Liu, Dan He, Xi Xia, Tao Zhu, Juncheng Wei, Peng Wu, Changyu Wang, Ling Xi, Qinglei Gao, Gang Chen, Rong Liu, Kezhen Li, Shuang Li, Shixuan Wang, Jianfeng Zhou, Ding Ma, Hui Wang 2015. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. J Clin Invest. 125, PubMedID: 25500889, DOI: 10.1172/JCI78206
- A-Bk Dad, S Ramakrishna, M Song, H Kim 2014. Enhanced gene disruption by programmable nucleases delivered by a minicircle vector. Gene Ther. , PubMedID: 25142139, DOI: 10.1038/gt.2014.76
Publications related to Genome Editing
Online ResourcesPlasmid Repositories:
CRISPR-gRNA Design Tools:
Genome Engineering using CRISPR/Cas Systems
Cas9-triggered homologous recombination
Drosophila RNAi Screening Center at Harvard Medical School
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Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.
CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro.