For the purposes of cloning, DNA assembly refers to a method to physically link together multiple fragments of DNA, in an end-to-end fashion, to achieve a desired, higher-order assembly prior to joining to a vector. This process is the cornerstone of the synthetic biology movement, and allows the construction of novel biological systems and devices using defined components. The methods are carried out in vitro, and are typically enzymatically driven with the final constructions being maintained in microbial host cells.
To help select the best DNA assembly method for your needs, please use our Synthetic Biology/DNA Assembly Selection Chart.
Learn more about the various DNA Assembly methods: NEBuilder HiFi DNA Assembly, Gibson Assembly, BioBrick Assembly, Golden Gate Assembly