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DNA Assembly and Cloning

For the purposes of cloning, DNA assembly refers to a method to physically link together multiple fragments of DNA, in an end-to-end fashion, to achieve a desired, higher-order assembly prior to joining to a vector. This process is the cornerstone of the synthetic biology movement, and allows the construction of novel biological systems and devices using defined components. The methods are carried out in vitro, and are typically enzymatically driven with the final constructions being maintained in microbial host cells.

To help select the best DNA assembly method for your needs, please use our Synthetic Biology/DNA Assembly Selection Chart.

Learn more about the various DNA Assembly methods: NEBuilder HiFi DNA Assembly, Gibson Assembly, BioBrick Assembly, Golden Gate Assembly

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DNA Assembly and Cloning includes these areas of focus:
NEBuilder® HiFi DNA Assembly
Gibson Assembly®
BioBrick® Assembly
Golden Gate Assembly
Protocols for DNA Assembly and Cloning
Application Notes for DNA Assembly and Cloning
    Publications related to DNA Assembly and Cloning
    • Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. (2019) Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc; 8 (29), e00721-19. PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
    • Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. (2018) Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synth Biol; 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
    • Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M (2014) A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants PLoS One; 9(2), e88218. PubMedID: 24551083, DOI: 10.1371/journal.pone.0088218
    • Feng Y, Zhang S, Huang X (2014) A robust TALENs system for highly efficient mammalian genome editing Sci Rep; 4, 3632. PubMedID: 24407151, DOI: 10.1038/srep03632
    • Abil Z, Denard CA, Zhao H (2014) Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA J Biol Eng; 8(1), 7. PubMedID: 24581042, DOI: 10.1186/1754-1611-8-7
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