
DNA Assembly and Cloning
Choose Type:
- Golden Gate Assembly Protocol for Using NEB Golden Gate Assembly Mix (E1600)
- Recommended Screening Protocols for Using NEB Golden Gate Assembly Mix (E1600)
- Transformation Protocol for Using NEB Golden Gate Assembly Mix (E1600)
- Golden Gate Assembly Protocol for Using NEB Golden Gate Assembly Kit (BsaI-HFv2) (E1601)
- Recommended Screening Protocols for Using NEB Golden Gate Assembly Kit (BsaI-HFv2)
- Transformation Protocol for Using NEB Golden Gate Assembly Kit (BsaI-HFv2) (E1601)
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How Gibson Assembly® is Changing Synthetic Biology
Understand how Gibson Assembly ® works and its impact in accelerating the progress of synthetic biology.
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Restriction Endonucleases: Molecular Cloning and Beyond
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Synthetic Genomics: Building a Better Bacterium
- Molecular Cloning Technical Guide
- Synthetic Biology/DNA Assembly Selection Chart
- PCR Troubleshooting Guide
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Expanded “assembly standards” for MoClo
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. 2019. Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc. 8 (29), PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
- Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. 2018. Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synthetic Biology. 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
- Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M 2014. A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants PLoS One. 9(2), PubMedID: 24551083, DOI: 10.1371/journal.pone.0088218
- Feng Y, Zhang S, Huang X 2014. A robust TALENs system for highly efficient mammalian genome editing Sci Rep. 4, PubMedID: 24407151, DOI: 10.1038/srep03632
- Abil Z, Denard CA, Zhao H 2014. Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA J Biol Eng. 8(1), PubMedID: 24581042, DOI: 10.1186/1754-1611-8-7
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