
DNA Assembly and Cloning
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- Golden Gate Assembly Protocol for Using NEB Golden Gate Assembly Mix (E1600)
- Recommended Screening Protocols for Using NEB Golden Gate Assembly Mix (E1600)
- Transformation Protocol for Using NEB Golden Gate Assembly Mix (E1600)
- Golden Gate Assembly Protocol for Using NEBridge Golden Gate Assembly Kit (BsaI-HFv2) (E1601)
- Recommended Screening Protocols for Using NEBridge Golden Gate Assembly Kit (BsaI-HFv2)
- Transformation Protocol for Using NEBridge Golden Gate Assembly Kit (BsaI-HFv2) (E1601)
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Synthetic Genomics: Building a Better Bacterium
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How Gibson Assembly® is Changing Synthetic Biology
Understand how Gibson Assembly ® works and its impact in accelerating the progress of synthetic biology.
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Restriction Endonucleases: Molecular Cloning and Beyond
- Molecular Cloning Technical Guide
- Synthetic Biology/DNA Assembly Selection Chart
- PCR Troubleshooting Guide
- Phage Display Troubleshooting Guide
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Expanded “assembly standards” for MoClo
Feature Articles
Brochures
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Anton, B.P., Morgan, R.D., Ezraty, B., Manta, B., Barras, F., Berkmen, M. (2019) Complete genome sequence of Escherichia coli BE104, an MC4100 drivative lacking the methionine reductive pathway Microbiol Resour Announc; 8 (29), e00721-19. PubMedID: 31296691, DOI: 10.1128/MRA.00721-19
- Potapov, V., Ong, J.L., Kucera, R.B., Langhorst, B.W., Bilotti, K., Pryor, J.M., Cantor, E.J., Canton, B., Knight, T.F., Evans, T.C., Lohman, G.J.S. (2018) Comprehensive profiling of four base overhang ligation fidelity by T4 DNA ligase and application to DNA assembly ACS Synth Biol; 7 (11), PubMedID: 30335370, DOI: 10.1021/acssynbio.8b00333
- Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske M (2014) A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants PLoS One; 9(2), e88218. PubMedID: 24551083, DOI: 10.1371/journal.pone.0088218
- Abil Z, Denard CA, Zhao H (2014) Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA J Biol Eng; 8(1), 7. PubMedID: 24581042, DOI: 10.1186/1754-1611-8-7
- Feng Y, Zhang S, Huang X (2014) A robust TALENs system for highly efficient mammalian genome editing Sci Rep; 4, 3632. PubMedID: 24407151, DOI: 10.1038/srep03632
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