SNAP-Surface® 594

SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells.

  • This is a cell impermeable substrate (BG-594) based on the ATTO-TEC dye ATTO 594
  • It is suitable for standard Texas Red and Alexa 594 filter sets
  • It has an excitation maximum at 606 nm and emission maximum at 626 nm

Ordering Information

  • 50 nmol
  • Product Information
    This package includes 50 nmol of SNAP-Surface 594 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on manmmalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Excitation (dotted line) and emission spectra of SNAP-Surface 594 coupled to SNAP-tag in buffer at pH 7.5 Figure 1: Excitation (dotted line) and emission spectra of SNAP-Surface 594 coupled to SNAP-tag in buffer at pH 7.5

    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling
    Snap Surface Products,
    In vivo Imaging,
    Pulse Chase
    Receptor Internalization
    Protein Localization
    Protein Labeling Snap Clip
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