This package includes
50 nmol of SNAP-Surface 649 substrate, sufficient
to make 10 ml of a 5 μM SNAP-tag fusion protein
labeling solution.
The SNAP-tag is a novel tool for protein research,
allowing the specific, covalent attachment of
virtually any molecule to a protein of interest. The
SNAP-tag is based on mammalian O6-alkylguanine-
DNA-alkyltransferase (AGT). SNAP-tag
substrates are derivatives of benzylguanines and
benzylchloropyrimidines. In the labeling reaction,
the substituted benzyl group of the substrate is
covalently attached to the SNAP-tag.
There are two steps to using this system: subcloning
and expression of the protein of interest
as a SNAP-tag fusion, and labeling of the fusion
with the SNAP-tag substrate of choice. Expression
of SNAP-tag fusion proteins is described in the
documentation supplied with SNAP-tag plasmids.
The labeling of the fusion proteins with the SNAPtag
substrate is described below.
Excitation (dotted line) and emission spectra of SNAP-Surface 649 coupled to SNAP-tag in buffer at pH 7.5Structure of SNAP-Surface 649 (MW 1221.3 g/mol).
This product is related to the following categories:
Cells expressing SNAP-fusion proteins. Proteins of interest can be expressed with the SNAP-tag as either an N- or a C-terminal fusion, but note that the tag needs to be exposed to the extracellular surface of the plasma membrane for labeling with SNAPSurface 649.
Storage: SNAP-Surface 649 should be stored at –20°C (longterm) or at 4°C in the dark (short term, less than4 weeks). Protect the substrate from light and moisture.With proper storage at –20°C the substrateshould be stable for at least three years dry or3 months dissolved in DMSO.
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938. For detailed information: see www.neb.com/cia/legal.
The products and/or their use may be covered by one or more of the following patents and patent applications: Methods for Using O6-Alkylguanine-DNA-Alkyltransferases: US 7,939,284; US 8,367,361; EP 1 410 023; EP 2 037 271; EP 1 696 234; EP 2 211 177; JP 4195815; JP 4226053; CN 1295510; CN 1975422; and SG 100125.
Substrates for O6-Alkylguanine-DNA Alkyltransferase: US 7,799,524; and JP 4976651.
Mutants of O6-Alkylguanine-DNA-Alkyltransferases: US 7,888,090. EP 1720981 (pending).
Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases: US 8,163,479. EP 1730298 (pending).
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