SNAP-Cell® 430

SNAP-Cell® 430 is a blue fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro.

  • It is a cell-permeable substrate (BG-430) based on diethylaminocoumarin
  • It is suitable for appropriate blue lasers and filter sets
  • It has an excitation maximum at 421 nm and emission maxima at 444 and 484 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package includes 50 nmol of SNAP-Cell 430 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on human O6-alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Figure 1: Live CHO-K1 expressing nuclear histone H2B-SNAP (H2B-SNAP) were labeled with SNAP-Cell™ 430 (aqua).

    Live CHO-K1 expressing nuclear histone H2B-SNAP (H2B-SNAP) were labeled with SNAP-Cell® 430 (aqua).
    Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 430 coupled to SNAP-tag in buffer at pH 7.5 Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 430 coupled to SNAP-tag in buffer at pH 7.5
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    Figure 3:  Structure of SNAP-Cell 430 (MW 513.5 g/mol).
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Cell,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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