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SNAP Cell

The SNAP-tag® system is based on a DNA repair enzyme, O6-alkylguanine-DNA alkyltransferase (AGT). It allows for multiple substrate options to enable color changes. It is highly temperature and fixation stable and can be used in vitro or in vivo.  The substrate consists of two parts; the benzylguanine group and the functional group, which can be a fluorophore, biotin or bead. During the labeling reaction, the substituted benzyl group covalently attaches to the SNAP-tag releasing free guanine. Once the fluorophore is coupled to the desired protein, the label fluoresces permitting visualization in living or fixed cells.

SNAP-tag® is a registered trademark of New England Biolabs, Inc.

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FAQs for SNAP Cell
Protocols for SNAP Cell
Application Notes for SNAP Cell
    Publications related to SNAP Cell
    • Meron Mengistu, Krishanu Ray, George K Lewis, Anthony L DeVico (2015) Antigenic properties of the human immunodeficiency virus envelope glycoprotein gp120 on virions bound to target cells PLoS Pathog; 11(3), e1004772. PubMedID: 25807494, DOI: 10.1371/journal.ppat.1004772.
    • Gabriele Fuchs, Alexey N Petrov, Caleb D Marceau, Lauren M Popov, Jin Chen, Sen E O'Leary, Richard Wang, Jan E Carette, Peter Sarnow, Joseph D Puglisi (2015) Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site Proc Natl Acad Sci U S A; 112(2), 319-325. PubMedID: 25516984, DOI: 10.1073/pnas.1421328111.
    • Margaret L Rodgers, Joshua Paulson, Aaron A Hoskins (2015) Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull RNA; 21(5), 1031-1041. PubMedID: 25805862, DOI: 10.1261/rna.047845.114
    • P, Katajisto., Dohla, J., Chaffer, CL., Pentinmikko, N., Marjanovic, N., Igbal, S., Zoncu, R., Chen, W., Weinberg, RA., Sabatini, DM. (2015) Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness Science; 348(6232), 340-343. PubMedID: 25837514, DOI: 10.1126/science.1260384.
    • Julien Mouysset, Samuel Gilberto, Michelle G Meier, Fabienne Lampert, Mukta Belwal, Patrick Meraldi, Matthias Peter (2015) CRL4RBBP7 is required for efficient CENP-A deposition at centromeres J Cell Sci; 128(9), 1732-1745. PubMedID: 25795299, DOI: 10.1242/jcs.162305.
    • Juri Nio Bach, Marc Bramkamp (2015) Dissecting the molecular properties of prokaryotic flotillins PLoS One; 10(1), e0116750. PubMedID: 25635948, DOI: 10.1371/journal.pone.0116750.
    • Domoszlai T, Martincuks A, Fahrenkamp D, Schmitz-Van de Leur H, Küster A, Müller-Newen G (2014) Consequences of the disease-related L78R mutation for dimerization and activity of STAT3 J Cell Sci; 127(Pt 9), 1899-910. PubMedID: 24569879, DOI: 10.1242/jcs.137422
Features
  • Clone and express once, then use with a variety of Substrates
  • Non-toxic to living cells
  • Wide selection of fluorescent substrates
  • Highly specific covalent labeling
  • Simultaneous dual labeling
Applications
  • Simultaneous dual protein labeling inside live cells
  • Protein localization and translocation
  • Pulse-chase experiments
  • Receptor internalization studies
  • Selective cell surface labeling
  • Protein pull-down assays
  • Protein detection in SDS-PAGE
  • Flow cytometry
  • High throughput binding assays in microtiter plates
  • Biosensor interaction experiments
  • FRET-based binding assays
  • Single molecule labeling
  • Super-resolution microscopy
Protein Labeling with SNAP-tag and CLIP-tag
The SNAP- (gold) or CLIP-tag (purple) is fused to the protein of interest (blue). Labeling occurs through covalent attachment to the tag, releasing either a guanine or a cytosine moiety.
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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