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SNAP-Cell® TMR-Star

SNAP-Cell® TMR-Star is a red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro.

  • It is a cell-permeable substrate (CP-6-TMR) is based on the single isomer 6-carboxytetramethylrhodamine
  • It is suitable for standard rhodamine filter sets
  • It has an excitation maximum at 554 nm and an emission maximum at 580 nm
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Catalog # Concentration Size List Price Your Price Quantity
S9105S Not Applicable 30 nmol $362.00
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  • Product Information
    This package contains 30 nmol of SNAP-Cell TMR-Star substrate, sufficient to make 10 ml of a 3 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1:  Live COS-7 expressing mitochondrial cytochrome oxidase 8A-SNAP (COX8A-SNAP) were labeled with SNAP-Cell TMR-Star (red). Nuclei were counterstained with Hoechst 33342 (blue).

    Live COS-7 expressing mitochondrial cytochrome oxidase 8A-SNAP (COX8A-SNAP) were labeled with SNAP-Cell TMR-Star (red). Nuclei were counterstained with Hoechst 33342 (blue).
    Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell TMR-Star after coupling to SNAP-tag in buffer at pH 7.5 Excitation (dotted line) and emission spectra of SNAP-Cell TMR-Star after coupling to SNAP-tag in buffer at pH 7.5

    Figure 3: Structure of SNAP-Cell TMR-Star (MW 677.1 g/mol)
    Figure 3: Structure of SNAP-Cell TMR-Star (MW 677.1 g/mol)
    This product is related to the following categories:
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    This product can be used in the following applications:
    SNAP Cell,
    In vivo Imaging,
    Pulse Chase,
    Receptor Internalization,
    Protein Localization,
    Protein Labeling Snap Clip
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