Restriction Endonucleases

The Leader in the Discovery and Production of Restriction Enzymes

After > 45 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

NEB feels that moving away from animal-containing products is a step in the right direction.  We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free! Learn more at


Choose Type:

Restriction Endonucleases includes these subcategories:
High-Fidelity (HF®) Restriction Endonucleases
Homing Endonucleases
Nicking Endonucleases
Restriction Enzymes for Epigenetic Analysis
Time-Saver Qualified Restriction Enzymes
Protocols for Restriction Endonucleases
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.